黄芪多糖对大菱鲆头肾巨噬细胞免疫激活机制的研究

doi:10.16378/j.cnki.1003-1111.24174

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Abstract In order to explore the immunomodulatory mechanism of Astragalus polysaccharide (APS) on macrophages, head kidney macrophages of 6—7-month-old turbot with body weight of (346.00±95.72) g were isolated in vitro, and 800 μg/mL of APS was used to treat the macrophages for 24 h, and the differential proteins and signaling pathways in the cells were analyzed by proteomics technology. The differentially expressed proteins and signaling pathways in the cells were analyzed using proteomics techniques, and molecular docking was used to evaluate the interaction between APS and pathway receptor proteins. The results showed that no cytotoxic effect, whereas improvement of cell growth to some extent, was observed in the turbot macrophages stimulated by APS at concentrations ranging from 50 to 1000 μg/m for 24 hours, with stimulation of macrophages to produce nitric oxide (NO) at the secretion stabilizing at concentrations above 800 μg/mL. Proteomics analysis revealed upregulation of proteins related to the TNF signaling pathway, PI3K-Akt signaling pathway, and NF-κB signaling pathway at 800 μg/mL. Molecular docking results indicated that APS interacted with Toll-like receptors, specifically TLR2, TLR3, and TLR22, forming one, two, and three hydrogen bonds, respectively. It is therefore speculated that TLR22 may be a core target for APS activation of the head kidney macrophages in turbot.In conclusion, this study preliminarily identifies that APS at 800 μg/mL activates the TNF, PI3K-Akt, and NF-κB signaling pathways to regulate cytokine expression, exerting an immune regulatory effect on the head kidney macrophages of turbot, with a possible involvement of TLR22 in the recognition of APS.

Key words： Scophthalmus maximus Astragalus polysaccharide macrophage immune regulation

Received: 14 October 2024

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S941.4

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	DONG Xufeng
	WANG Xinyue
	WANG Xiaoxue
	GUO Pei
	QIN Zhihua

Cite this article:

DONG Xufeng,WANG Xinyue,WANG Xiaoxue, et al. Immunoregulatory Effect of Astragalus Polysaccharide on Head Kidney Macrophages of Turbot[J]. Fisheries Science, 2025, 44(5): 737-746.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24174 OR https://www.shchkx.com/EN/Y2025/V44/I5/737

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