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| Construction of Different Core Breeding Populations of Kelp Saccharina japonica Based on SSR Markers |
| LI Yan1,2, LIU Yanling1,3, LIANG Guangjin1,2, WANG Liqin1,2, LI Xiaojie1,2, ZHAO Juping1,2, WU Ruina1,3, TIAN Pingping1,3 |
1. Shandong Oriental Ocean Sci-Tech Co., Ltd, Yantai 264003, China;
2. Shandong Technology Innovation Center of Algae and Sea Cucumber, Yantai 264003, China;
3. Provincial Key Laboratory of Marine Seed Industry of Shandong, Yantai 264003, China |
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Abstract In order to improve the accuracy of kelp Saccharina japonica preservation, a method for constructing the core breeding populations of the kelp with diverse phenotypes and comprehensive genetic information was proposed for the first time by utilizing the microsatellite molecular markers of the kelp, and applied in four distinct lines. A total of 153 SSR markers from the kelp microsatellite marker library were used in the DFKZ-3,DFXZ-3,DFXZ-1,and DFXZ-2 breeding populations and 15 loci with good polymorphism were screened out from each line to analyze the genetic diversity of the population. After a comprehensive evaluation of allelic weight scores, individual combination scores, species status, and other relevant factors, the final number of preserved individuals in the DFKZ-3, DFXZ-3, DFXZ-1, and DFXZ-2 kelp lines was determined as 12, 12, 11, and 17, respectively. Germplasm collection and separation were carried out when the sporangium was mature according to the number of the preserved kelp, and gametophytes were retained for seed preservation, so as to build a core breeding population, which laid a foundation for the sustainable utilization of improved varieties, further development of germplasm resources and screening of more core kelp seed banks.
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Received: 13 September 2024
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