钝吻黄盖鲽染色体核型及带型特征

doi:10.16378/j.cnki.1003-1111.25033

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Abstract Marbeled flounder Pseudopleuronectes yokohamae is an important indigenous marine economic fish species in China, and its genetic characteristics remain incompletely understood. To investigate the chromosomal karyotype and banding patterns of P. yokohamae, this study selected 50 individuals (3—4 months old, total length 3—5 cm) as experimental materials. The karyotype of P. yokohamae was studied through the chromosome preparations obtained from dorsal fin tissue by the method of soak in colchicines. Subsequently, the chromosome samples were prepared using a low permeation and Carnoy′s fixation air drying method. We investigated the banding features and morphological characteristics of multiple chromosomes banding pattern (C-banding, Ag-NORs and G-banding). The species exhibits a typical telocentric chromosomal composition, with a karyotype formula of 2n=48t and a chromosomal arm number (NF) of 48. No polyploidy, sex chromosomes, or satellite chromosomes were detected. In the C-banding characteristics, the whole 17th pair of chromosomes bored positive C-banding, the 9th pair of chromosomes showed negative result and the first three pairs of homologous chromosomes exhibited telomeric C-banding, the rest were centromere C-banding. According to the formula, the amount of heretochromatin calculated was 32.05%. P. yokohamae beared a pair of Ag-NORs which was located at the end of long arm of the 2nd chromosome.The size and location of G-banding were same in homologous chromosomes, but they were not same in the non-homologous chromosomes. Statistics showed that there were 58 deeply stained bands and 38 slightly stained bands in the chromosome of P. yokohamae, and the 11th pair of chromosomes was slightly stained band. The findings would provide basic data for the genomic research, the origin of the species, the status of evolution, and the classification of the organisms.

Key words： Pseudopleuronectes yokohamae chromosome karyotype analysis C-banding Ag-NORs G-banding

Received: 14 February 2025

PACS:

Q959.4

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	Articles by authors
	ZHAO Zihan
	CUI Aijun
	ZHANG Ning
	LIU Xuezhou
	XU Yongjiang
	ZHAO Rixiang
	SUN Zongzhe

Cite this article:

ZHAO Zihan,CUI Aijun,ZHANG Ning, et al. Analysis of the Karyotype and Banding Patterns of Marbeled Flounder Pseudopleuronectes yokohamae[J]. Fisheries Science, 2025, 44(5): 727-736.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.25033 OR https://www.shchkx.com/EN/Y2025/V44/I5/727

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