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| Effect of Salinity on Alkaline Potassium Persulfate Oxidation Method for Determination of Total Nitrogen Analysis |
| YIN Zhijian, JIANG Chengcheng, LIU Jianlong, WANG Shiwei, WANG Lianshun, CONG Yuting, WANG Li, WANG Hua, WANG Yuan, LU Yanan, YANG Guojun |
1. National Demonstration Center for Experimental Aquaculture Education, Ministry of Education, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;
2. Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China;
3. Dalian Key Laboratory of Breeding, Reproduction and Aquaculture of Crustaceans, Dalian 116023, China |
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Abstract This study systematically explored the applicable salinity range of seawater total nitrogen analysis method [alkaline potassium persulfate oxidation method (GB 17378.4—2007)] and water quality total nitrogen analysis method [alkaline potassium persulfate oxidation-ultraviolet spectrophotometry (HJ 636—2012)] and the reason why water quality total nitrogen analysis method could not test high salinity samples. In the test, the applicable salinity range of the two total nitrogen analysis methods was determined by comparing the blank value, r2, and nitrate reduction rate of the eight different standard curves of the two water quality analysis methods in the salinity range from 0 to 35. The results showed that the seawater total nitrogen analysis method could detect seawater samples with different salinity gradients, and with the recovery rate from 92% to 100%. The total nitrogen analysis method can only detect seawater samples with salinity ≤20, with the recovery rate from 92% to 106%. At the salinity of over 20, there is potassium persulfate residue in the sample using the water quality total nitrogen analysis method. Subsequently, the potassium persulfate residual concentration of the total nitrogen analysis method of water quality at different salinities was compared by the potassium persulfate residual experiment and acetic acid titration experiment to verify why the potassium persulfate oxidation method could not determine high salinity. Finally, the total nitrogen analysis method of seawater and the total nitrogen analysis method of water quality are compared to detect seawater samples to determine whether the dilution method can detect seawater samples. In addition, the water quality total nitrogen analysis method cannot detect high-salinity seawater diluted samples. The finding systematically analyzes the applicable salinity range of the two total nitrogen analysis methods, explains why the water quality total nitrogen analysis method can not detect high-salinity samples, and provides a reference and basis for the detection of total nitrogen index and method improvement in the future.
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Received: 11 May 2024
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