Karyotype and Ploidy Analysis of Gymnocypris selincuoensis
LI Xin1, MA Kexin1, GUO Wenxuan1, LI Yifan1, YANG Yueyao1, ZHOU Ziyu1, LIU Haiping2, XIAO Shijun3, ZHOU He1
1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2. Institute of Fisheries Science, Xizang Autonomous Region Academy of Agriculture and Animal Husbandry Sciences, Lhasa 850000, China; 3. Jiaxing Key Laboratory for New Germplasm Breeding of Economic Mycology, Jiaxing 314000, China
Abstract：Harsh plateau environment in Tibetan Plateau has unique and rich biological resources. The chromosomes in different groups and genera in the subfamily Schizothoracinae showed diverse characteristics.The chromosome samples were prepared from renal tissue cells of Gymnocypris selincuoensis with body weight of 246.0—374.2 g by the conventional cold drop method. Giemsa staining analysis was used to determine the chromosome number and karyotype. The band type and ploidy were speculated through Ag-NORs and Chromomycin A3/4,6-diamidino-2-phenylindole double fluorescent staining technology. It was found that the karyotype formula of G. selincuoensis was 4n=88, 40m+20sm+28st/t, NF=148. In interphase nucleoli, the maximal frequency of Ag-NORs sites was 4. In each metaphase karyotype, both Ag-NORs sites and CMA3-positive sites were shown to be 4 on the short arms of submetacentric chromosomes. In conclusion, different G. selincuoensis samples had identical chromosome numbers. G. selincuoensis is a genetic tetraploid with four sets of chromosomes. The finding provides an important basis for studying genetic relationships among species and reference for the subsequent research on the classification and evolutionary status of the subfamily Schizothoracinae.
[1] 张春光,许涛清,蔡斌,等.西藏鱼类的组成分布及渔业区划[J].西藏科技,1996(1):10-19. [2] 陈毅峰.裂腹鱼类的系统进化及资料生物学[D].武汉:中国科学院水生生物研究所,2000:21-82. [3] 张驰,李宝海,周建设,等.西藏渔业资源保护现状、问题及对策[J].水产学杂志,2014,27(2):68-72. [4] 武云飞,康斌,门强,等.西藏鱼类染色体多样性的研究[J].动物学研究,1999,20(4):258-264. [5] 史建全,杨建新,祁洪芳,等.青海湖裸鲤形态特征与遗传性状[J].青海科技,2000,7(2):16-18. [6] FENG X, JIA Y T, ZHU R, et al. Characterization and analysis of the transcriptome in Gymnocypris selincuoensis on the Qinghai-Tibetan Plateau using single-molecule long-read sequencing and RNA-seq[J]. DNA Research,2019,26(4):353-363. [7] 陈毅峰,何德奎,蔡斌.色林错渔业生产的现状与可持续利用的对策[J].生物多样性,2001,9(1):85-89. [8] 陈毅峰,何德奎,陈宜瑜.色林错模式与青藏高原湖泊的可持续发展[G] //中国科学技术协会,中国土木工程学会.西部大开发 科教先行与可持续发展——中国科协2000年学术年会文集:2000年卷.北京:中国科学技术出版社,2000:305. [9] 陈毅峰,何德奎,曹文宣,等.色林错裸鲤的生长[J].动物学报,2002,48(5):667-676. [10] 刘军.色林错裸鲤生活史类型的模糊聚类分析[J].水利渔业,2006,27(2):17-18. [11] 陈毅峰,何德奎,陈宜瑜.色林错裸鲤的年龄鉴定[J].动物学报,2002,48(4):527-533. [12] 何德奎,陈毅峰,陈自明,等.色林错裸鲤性腺发育的组织学研究[J].水产学报,2001,25(2):97-102. [13] 周贺,魏杰,蔡明夷.水产动物染色体分析技术及应用[M].北京:中国农业出版社,2022. [14] LEVAN A, FREDGA K, SANDBERG A A. Nomenclature for centromeric position on chromosomes[J]. Hereditas,2009,52(2):201-220. [15] HOWELL W M, BLACK D A. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer:a 1-step method[J]. Experientia,1980,36(8):1014-1015. [16] SCHWEIZER D. Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes[J]. Cytogenetics and Cell Genetics,1980,27(2/3):190-193. [17] SCHWEIZER D, AMBROS P, ANDRLE M. Modification of DAPI banding on human chromosomes by prestaining with a DNA-binding oligopeptide antibiotic, distamycin A[J]. Experimental Cell Research,1978,111(2):327-332. [18] AMORES A, MARTINEZ G, REINA J, et al. Karyotype, C-banding, and Ag-NOR analysis in Diplodus bellottii (Sparidae, Perciforms). Intra-individual polymorphism involving heterochromatic regions[J]. Genome,1993,36(4):672-675. [19] 曾庆凯,杜合军,杨菁.鲟形目鱼类染色体研究现状及进展[J].水产科学,2019,38(5):734-740. [20] KHUDA-BUKHSH A R, NAYAK K. Karyomorphological studies in two species of hillstream fishes from Kashmir,India:occurrence of a high number of chromosomes[J].Chromosome Information Services,1982(33):12-14. [21] 昝瑞光,刘万国,宋峥.裂腹鱼亚科中的四倍体-六倍体相互关系[J].遗传学报,1985,12(2):137-142. [22] 山谷,郭文轩,李信,等.纳木错裸鲤染色体组的构成及倍性分析[J].大连海洋大学学报,2022,37(2):251-257. [23] 余先觉,周暾,李渝成,等.中国淡水鱼类染色体[M].北京:科学出版社,1989:152-153. [24] 马凯,佟广香,张永泉,等.尖裸鲤染色体核型分析及进化地位研究[J].西北农林科技大学学报(自然科学版),2021,49(12):28-33. [25] 闫学春,史建全,孙效文,等.青海湖裸鲤的核型研究[J].东北农业大学学报,2007,38(5):645-648. [26] WU C Z, WU Y F, LEI Y L, et al. Studies on the karyotypes of four species of fishes from the Mount Qomolangma Region in China[C] //LI D S. Proceedings of the international Symposium on Aquaculture. Qingdao: Qingdao Ocean University Press, 1996:95-103. [27] 邹习俊.四川裂腹鱼核型及遗传多样性研究[D].贵阳:贵州大学,2009:27-30. [28] 陈永祥.四川裂腹鱼(Schizothorax kozlovi Nikolsky)种质特征及其遗传多样性研究[D].雅安:四川农业大学,2013:65-73. [29] 余祥勇,李渝成,周暾.中国鲤科鱼类染色体组型研究——8种裂腹鱼亚科鱼类核型研究[J].武汉大学学报(自然科学版),1990,36(2):97-104. [30] 李渝成,李康,桂建芳,等.中国鲤科鱼类染色体组型的研究Ⅺ.裂腹鱼亚科二种鱼和鳅鮀亚科三种鱼的染色体组型[J].水生生物学报,1987,11(2):184-186. [31] 代应贵,肖海.裂腹鱼类种质多样性研究综述[J].中国农学通报,2011,27(32):38-46. [32] 周建设,曾本和,王且鲁,等.不同海拔拉萨裸裂尻线粒体16S rRNA基因序列变异及遗传多样性分析[J].水产科技情报,2019,46(3):130-134. [33] 杨志刚,杜军,林志强.1961—2012年西藏色林错流域极端气温事件变化趋势[J].生态学报,2015,35(3):613-621. [34] 刘海平,牟振波,蔡斌,等.供给侧改革与科技创新耦合助推西藏渔业资源养护[J].湖泊科学,2018,30(1):266-278. [35] RISHI K K, SINGH J, KAUL M M. Chromosomal analysis of Schizothoraichthys progastus, (McClelland)(Cyprinids:Cypriniformes)[J]. Chromosome Information Services,1983(34):12-13. [36] 王鑫,马浩,祝东梅,等.杂交鲌 “先锋1号” 染色体组型分析和DNA含量测定[J].水产科学,2021,40(3):347-353. [37] 袁秀丹.多倍体鲫鲤荧光定量内参基因的筛选及其表达稳定性分析[D].长沙:湖南师范大学,2021:1-3. [38] 刘声宇,张文龙,林声雄.泥鳅多倍体研究进展[J].现代农业科技,2018(9):245-246. [39] 曹柳.鲫鲂杂交品系的rRNA基因的染色体位点及组成分析[D].长沙:湖南师范大学,2019:7-9. [40] 李志,鲁蒙,周莉,等.外源精子基因组整入雌核生殖银鲫卵子创制异源八倍体的有效方法[J].水生生物学报,2020,44(1):26-35. [41] 桂建芳,李渝成,李康,等.中国鲤科鱼类染色体组型的研究Ⅵ:50亚科3种四倍体鱼和鲤亚科1种四倍体鱼的核型[J].遗传学报,1985,12(4):302-308. [42] 李树深.云南湖泊鱼类的区系及其类型分化[J].动物学报,1982,28(2):169-176. [43] 王绪祯,甘小妮,李俊兵,等.鲤亚科多倍体物种独立起源及其与第三纪青藏高原隆升的关系[J].中国科学(生命科学),2016,46(11):1277-1295. [44] 毛慧玲,葛欣琦,刘佳丽,等.鄱阳湖黄颡鱼染色体核型分析及进化地位探讨[J].江西农业大学学报,2012,34(6):1222-1225. [45] 小岛吉雄.鱼类细胞遗传学[M].林义浩编译.广州:广东科技出版社,1991:8-33. [46] 李树深.鱼类细胞分类学[J].生物科学动态,1981:2,8-15. [47] 曹文宣,陈宜瑜,武云飞,等.裂腹鱼类的起源和演化及其与青藏高原隆起的关系[M].北京:科学出版社,1981:118-130. [48] 武云飞,谭齐佳.青藏高原鱼类区系特征及其形成的地史原因分析[J].动物学报(英文版),1991,37(2):135-152. [49] 陈宜瑜,陈毅峰,刘焕章.青藏高原动物地理区的地位和东部界线问题[J].水生生物学报,1996,20(2):97-103.
2024, Vol. 43 
