Effects of Salinity Regulation Methods on Establishment of Marine Biological Flocculation Culture System
WU Huifang1, LUO Guozhi1,2,3, TAN Hongxin1,2,3, MENG Haoyan1
1.Research and Development Center of Aquaculture Engineering of Shanghai, Shanghai 201306, China; 2.NationalDemonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; 3.Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding, Shanghai 201306, China
Abstract：Effects of three salinity addition methods on culture flocs and the start-up efficiency of bioflocculation system were investigated in nine cylindrical aquaculture buckets with a volume of 100 L and with eel feed as raw materials, in which salinity was adjusted to 30 at the beginning of the experiment in the first group, the salinity was increased by 5 degrees every day after the end of the depression, adding 1 degree every 3 hours in the second group, and the salt was added into cultured freshwater biological flocculation system according to the way of adding salt in the second group in the third group. After startup, the removal efficiency of 10 mg/L ammonia nitrogen in three treatment groups was monitored.The results showed that the contents of ammonia nitrogen and nitrite nitrogen in the second and third groups were decreased to low level before the salinity was adjusted to 30 in first group. During the culture period, there was better settleability of flocs in the second and third groups than that in the first group. The high-throughput sequencing revealed that Flavobacteria was the dominant class in the biological flocculation system with salinity of 30, and Sphingobacteria was the dominant class in the flocculation system with initial salinity of 30. Actinomycetes is the dominant class in the biological flocculation system of freshwater culture and redomestication into seawater. Leptobactrrium and norank_f_Segniliparacea were the dominant genus of marine biological flocculation system with salinity of 30.It was concluded that the second salt regulation method was the most beneficial to cultivate marine biological flocculation system combined with the continuous monitoring of the removal effect of ammonia nitrogen in the three treatment groups after start-up, without significant difference (P>0.05).
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2020, Vol. 39 
