Effects of Storage Temperature and Humidity on Enzyme Activities and Strength of Agar Gel in Brown Alga Gracilaria
ZHANG Xiwen1, WU Ling1,2,3,4, TONG Fengjing1, JIANG Zedong1,2,3,4, ZHU Yanbing1,2,3,4, XIAO Anfeng1,2,3,4, NI Hui1,2,3,4, LI Qingbiao1,2,3,4
1. College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; 2. Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Technology, Xiamen 361021, China; 3. Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed, Xiamen Southern Ocean Technology Center of China, Xiamen 361021, China; 4. Research Center of Food Biotechnology of Xiamen City, Xiamen 361021, China
Abstract:Brown alga Gracilaria samples were disposed in a fresh-keeping box and thus had moisture of 10%, 14%, 40%, and 60% by adjusting the humidity in the fresh-keeping box. Then the samples with different moistures were continuously treated in a constant temperature incubator at 5 ℃, 15 ℃, 25 ℃, and 35 ℃ for 4 days, and agarase enzyme activity, reducing sugar content, agar extract extraction yield, agar gel strength, and other indicators were measured daily after treatment, three times in each group to clarify the intrinsic relationship between agarase activity and agar gel strength. The results showed that the changes in ambient temperature and humidity led to have an important influence on the activity of agaric enzyme and the strength of the extracted agar gel, with humidity being the most important factor. There were increases in agarase activity and the gel strength of agar as the humidity was elevated in the brown alga. There was stronger gel strength in the agar extracted from the brown alga containing 10% moisture 1 day and 2 days after the start of storage, with the maximum gel strength of (604.2±136.7), than that in gel extracted from the brown alga containing moisture of 60%.
张曦文, 伍菱, 童风景, 姜泽东, 朱艳冰, 肖安风, 倪辉, 李清彪. 贮藏条件对江蓠琼胶酶活性和琼胶凝胶强度的影响[J]. 水产科学, 2020, 39(6): 880-886.
ZHANG Xiwen, WU Ling, TONG Fengjing, JIANG Zedong, ZHU Yanbing, XIAO Anfeng, NI Hui, LI Qingbiao. Effects of Storage Temperature and Humidity on Enzyme Activities and Strength of Agar Gel in Brown Alga Gracilaria. Fisheries Science, 2020, 39(6): 880-886.
[1]李永梅,刘瑞,杨楠,等. 海南省4种江蓠属(红藻门)海藻的形态分类学研究[J]. 热带海洋学报,2018,37(4):29-37. [2]许忠能,林小涛. 江蓠的资源与利用[J]. 中草药,2001,32(7):654-657. [3]Sun X N, Duan M M, Liu Y L, et al. The beneficial effects of Gracilaria lemaneiformis polysaccharides on obesity and the gut microbiota in high fat diet-fed mice[J]. Journal of Functional Foods,2018,46:48-56. [4]吴湛霞,董静静,李思东,等. 国内江蓠提取琼胶加工工艺的研究进展[J]. 广西轻工业,2010,26(9):19-20. [5]姜绪,李佳蕙,李斌,等. 高温条件下营养盐对江蓠生长与氮、磷去除效率的影响[J]. 水产科学,2019,38(1):19-25. [6]赵谋明. 江蓠琼胶加工中碱处理的作用及机理[J]. 食品科学,1991(11):14-17. [7]刘名求,杨贤庆,戚勃,等.江蓠活性多糖与藻胆蛋白的研究现状与展望[J].食品工业科技,2013,34(13):338-341. [8]Ben Said R, Mensi F, Majdoub H, et al. Effects of depth and initial fragment weights of Gracilaria gracilis on the growth, agar yield, quality, and biochemical composition[J]. Journal of Applied Phycology,2018,30(4):2499-2512. [9]穆凯峰,吴永沛,陈昭华,等. 坛紫菜琼胶生产工艺的研究[J]. 水产科学,2009,28(8):454-457. [10]贺丽虹,吴汪黔生,沈颂东,等. 盐度对细基江蓠繁枝变型的生长、琼胶产量和组成的影响[J]. 海洋通报,2002,21(3):39-43. [11]Xiao Q, Weng H F, Chen G, et al. Preparation and characterization of octenyl succinic anhydride modified agarose derivative[J]. Food Chemistry,2019,279:30-39. [12]Enoki T, Okuda S, Kudo Y, et al. Oligosaccharides from agar inhibit pro-inflammatory mediator release by inducing heme oxygenase 1[J]. Bioscience, Biotechnology, and Biochemistry,2010,74(4):766-770. [13]Zarrintaj P, Manouchehri S, Ahmadi Z, et al. Agarose-based biomaterials for tissue engineering[J]. Carbohydrate Polymers,2018,187:66-84. [14]李苗苗. 人体肠道微生物对琼胶与κ-卡拉胶及其寡糖的降解和利用研究[D]. 青岛:中国海洋大学,2014. [15]Chhatbar M U, Godiya C B, Siddhanta A K. Functional modification of agarose: a facile synthesis of an agarose-saccharate derivative[J]. Carbohydrate Polymers,2012,88(3):1118-1123. [16]缪伏荣,李忠荣. 琼胶的降解及其产物的开发应用[J]. 现代农业科技,2007(2):125. [17]Chew K W, Show P L, Yap Y J, et al. Sonication and grinding pre-treatments on Gelidium amansii seaweed for the extraction and characterization of agarose[J]. Frontiers of Environmental Science & Engineering,2018,12(4):2. [18]李琴梅,戚勃.琼脂的物化特性及其在食品工业中的应用[J].中国食品添加剂,2009(6):170-174. [19]Armisen R. World-wide use and importance of Gracilaria[J]. Journal of Applied Phycology,1995,7(3): 231-243. [20]刘施琳,朱丰,林圣楠,等. 琼脂凝胶强度及弛豫特性的研究[J]. 食品工业科技,2017,38(13):85-89. [21]杨华,庄陈丰.响应面法优化微波辅助提取龙须菜多糖工艺及其抗氧化活性研究[J].食品科学,2011,32(20):79-83. [22]王连杰,沈照鹏,穆惠敏,等.微波法提取龙须菜琼胶及其理化性质的研究[J].水产学报,2017,41(6):854-860. [23]宗培杰,赵镜锟,张晓东. 从江蓠藻中提取琼胶的工艺研究[J]. 青岛大学学报:工程技术版,2016,31(2):89-94. [24]Freile-Pelegrín Y, Murano E. Agars from three species of Gracilaria (Rhodophyta) from Yucatán Peninsula[J]. Bioresource Technology,2005,96(3):295-302. [25]Freile-Pelegrín Y, Robledo D. Influence of alkali treatment on agar from Gracilaria cornea from Yucatán, México[J]. Journal of Applied Phycology,1997,9(6):533-539. [26]Marinho-Soriano E, Silva T S, Moreira W S. Seasonal variation in the biomass and agar yield from Gracilaria cervicornis and Hydropuntia cornea from Brazil[J]. Bioresource Technology,2001,77(2):115-120. [27]Marinho-Soriano E, Bourret E. Effects of season on the yield and quality of agar from Gracilaria species (Gracilariaceae, Rhodophyta)[J]. Bioresource Technology,2003,90(3):329-333. [28]Orduña-Rojas J, García-Camacho K Y, Orozco-meyer P, et al. Agar properties of two species of Gracilariaceae from the Gulf of California, Mexico[J]. Journal of Applied Phycology,2008,20(2):169-175. [29]Freile-Pelegrín Y. Does storage time influence yield and agar properties in the tropical agarophyte Gracilaria cornea?[J]. Journal of Applied Phycology,2000,12(2):153-158. [30]张惟杰. 复合多糖生化研究技术[M]. 上海:上海科学技术出版社,1987. [31]肖琼,肖安风,姚德恒,等.响应面法优化琼胶的酶解条件[J].中国食品学报,2016,16(7):140-148. [32]Romero J B, Villanueva R D, MontanO M N E. Stability of agar in the seaweed Gracilaria eucheumatoides (Gracilariales, Rhodophyta) during postharvest storage[J]. Bioresource Technology,2008,99(17):8151-8155. [33]Bird K T, Hanisak M D, Ryther J. Chemical quality and production of agars extracted from Gracilaria tikvahiae grown in different nitrogen enrichment conditions[J]. Botanica Marina,1981,24(8):441-444. [34]Romero J B, Montano M N E, Merca F, et al. Effect of sucrose on some physical properties of different Philippine agars[J]. The Philippine Journal of Science,2000,129(1):7-13. [35]Kapraun D F. Red algal polysaccharide industry: economics and research status at the turn of the century[J]. Hydrobiologia,1999,398/399:7-14. [36]王艳. 中长链脂肪酸淀粉酯的酶法合成及其性质研究[D].哈尔滨:哈尔滨商业大学,2013.