Effects of Microcystis aeruginosa on Reproduction and Activities of Antioxidant Enzymes of Water Fleas Moina mongolica
XING Hao1, CHEN Taoying2, DUAN Yuanliang1, GAO Xiaofeng1, LIU Wei1, ZHONG Yiyun1, ZHANG Jianlin1, CHANG Jianan1, SUN Bin1, HE Peimin1
1. Shanghai University Engineering Research Center for Water Environment Ecology, School of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China; 2. Aquatic Ecology Laboratory, School of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China
Abstract：With the eutrophication of water bodies and global warming, microcystis blooms often occur in low-salinity enclosed sea areas. In order to explore the important effects of the toxicity of Microcystis aeruginosa in low-salinity enclosed seas on the reproduction and antioxidant enzymes of saltwater cladocerans, the toxic M. aeruginosa with a salt tolerance of 8 was cultivated under indoor conditions at different development stages. Density of toxic M. aeruginosa [4 toxic M. aeruginosa treatment groups, 1 control group (Chlorella sp.), and 1 fasting group], mixed foods of different combinations (set 4 groups and 1 control group, with an alga density of 1×105 ind./mL) on the growth and reproduction of water fleas Moina mongolica, and the effects of different food conditions on the activities of superoxide dismutase (SOD) and catalase (CAT) in M.mongolica.The results showed that under different alga density conditions, M. mongolica had significant reduce in lifespan as the density of toxic M. aeruginosa increased, and there was no reproduction in the toxic M. aeruginosa treatment group. Under the condition of mixed food (Chlorella sp. + toxic M. aeruginosa), the greater the proportion of toxic M. aeruginosa, the lower the net reproductive rate of M. mongolica, the shorter the generation time; in the 96-hour enzyme activity test, compared with the Chlorella sp. group, the superoxide dismutase and catalase activities of M. mongolica exposed to the toxic M. aeruginosa was significantly decreased over time. Therefore, it was found that the toxic M. aeruginosa significantly inhibited the life history characteristics of M. mongolica. Under the two food conditions, the addition of Chlorella sp. can alleviate the toxic effect of M. aeruginosa on M. mongolica. At the same time, the toxicity of M. aeruginosa also significantly affected the superoxide dismutase and catalase activities of M. mongolica.
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2022, Vol. 41 
