Acute Toxicity of Thiamethoxam and Activities of Antioxidant Enzymes and Acetylcholinesterase in Juvenile Crucian Carp Carassius auratus
WANG Zhao1,2, PENG Shu1, MENG Lixia1
1. School of Life and Health Science, Kaili University, Kaili 556011, China; 2. Guizhou Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China
Abstract：Acute toxicity of thiamethoxam to juvenile crucian carp Carassius auratus with body weight of (19.81±2.40) g was investigated at water temperature of (24±1) ℃, pH 7.0±0.5, dissolved oxygen level of (5±1) mg/L, and thiamethoxam dilution to 24.35, 28.73, 33.90, 40.00 and 47.20 mg/L with acetone as the control by a semi-static toxicity method, and the effects of different concentrations (1 and 10 mg/L) of thiamethoxam on the activities of superoxide dismutase (SOD), catalase (CAT) and acetylcholinesterase (AChE) in different tissues of crucian carp was determined to understand the effect of thiamethoxam on the biochemical indices of crucian carp juveniles. It was found that the median lethal concentration of thiamethoxam to the juvenile was 39.22 mg/L in 24 h, 35.84 mg/L in 48 h, 30.71 mg/L in 72 h, and 28.95 mg/L in 96 h. There was increase in activities of SOD and CAT in the liver and gills first, and decrease then in the crucian carp juveniles exposed to 1 and 10 mg/L of thiamethoxam. The activity of AChE in the brain was varied with treatment period, wholly induced first and then inhibited. The findings indicated that thiamethoxam caused the dysfunction of fish antioxidant defense and neural system, thereby affecting the normal growth performance of fish.
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2020, Vol. 39 
