Strontium Labeling Technique of Otolith in Roughskin Sculpin Trachidermus fasciatus Based on Immersion Method
YANG Zuchang1, HU Wangjiao1, FENG Guangpeng1, ZHANG Hui1, ZHENG Yueping2, JI Qiang3
1. Shanghai Yangtze River Estuary Fishery Resources Proliferation and Ecological Restoration Engineering Technology Research Center, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; 2. Shanghai Aquatic Wildlife Conservation and Research Center, Shanghai 202162, China; 3. Shanghai Songjiang Fisheries Technical Extension Station, Shanghai 201699, China
Abstract：In order to probe the application of otolithic microchemical labeling technology in breeding and release of roughskin sculpin Trachidermus fasciatus, roughskin sculpin with body length of (8.34±0.77) cm and body weight of (7.89±2.57) g was reared in the third stage filtrated water containing SrCl2·H2O at concentrations of 0, 12, 18, 36 and 72 mg/L for 7 days at water temperature of (28.0±3) ℃ to explore the effect of different concentrations of strontium on the strontium labeling effect of otolites in roughskin sculpin. The results showed that there was no significant difference in mortality and growth in roughskin sculpin exposed to different concentrations of strontium labeling. There was an obvious linear relationship between the concentration of exogenous strontium and the ratio of Sr/Ca at the edge of otolith. With the increase in strontium concentration, the labeled Sr/Ca value was gradually increased, and the labeling effect increased significantly. The contents of strontium in muscle and gill were found to be a trend of first increasing and then decreasing with the change of time after labeling, with complete metabolism of the residual strontium in muscle within 30 days after labeling. The findings indicated that the strontium labeling principle and method were reliable and safe in the large-scale release population of roughskin sculpin. It was recommended that 36 mg/L be of the optimal labeling dose for exogenous strontium for 7 days labeling period.
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2025, Vol. 44 
