Removal of Nitrogen and Phosphorus from Mariculture Effluent by Bacillus velezensis BMF03
CHEN Zehui 1,2, JIANG Shengling1,2, CAI Yuefeng1,2,3, JI Nanjing 1,2,3, SHEN Xin1,2,3
1. School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China; 2. Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Lianyungang 222005, China; 3. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, China
Abstract：In order to investigate the removal effect of Bacillus velezensis BMF03 on nitrogen and phosphorus in simulated aquaculture wastewater, the B. velezensis BMF03 was inoculated into the simulated aquaculture wastewater prepared based on ammonium chloride, sodium nitrite, and dibasic sodium phosphate at initial density of 3.5×103, 3.5×104, 3.5×105, 3.5×106, 3.5×107 and 3.5×108 cfu/mL at the anaerobic culture without light at 28 ℃ for 48 h. The purification effect was comparatively evaluated between the screened optimal initial inoculum density of BMF03 and commercially available mixed Bacillus subtilis (3.5×107 cfu/mL). The results showed that B. velezensis BMF03 grew well in simulated aquaculture wastewater under different initial inoculum densities and effectively removed nutrients including ammonia nitrogen, nitrite nitrogen, and phosphate from the wastewater, with the removal rates of 100% for ammonia nitrogen and nitrite nitrogen in the 3.5×106—3.5×108 cfu/mL groups, significantly higher than that in the other groups (P<0.05). The total nitrogen removal rate was found to be 54.00% in the 3.5×107 and 50.34% in the 3.5×108 cfu/mL groups, and the phosphate removal rates to be 91.17% and 90.91%, both significantly higher than that in the other groups (P<0.05), and with the maximal removal rate of total phosphorus in the 3.5×107 cfu/mL group in 48 hours after inoculum (P<0.05). There was better removal effect on phosphate in the initial inoculum density of B. velezensis BMF03 at 3.5×105 cfu/mL than that in the mixed B. subtilis (P<0.05) compared with commercially available mixed B. subtilis (3.5×107 cfu/mL). The removal efficiency of ammonia nitrogen was shown to be significantly better than that in mixed B. subtilis (P<0.05) at initial inoculum density of 3.5×106 cfu/mL; and the removal efficiency of total nitrogen and total phosphorus by the bacteria was found to be better than that in mixed B. subtilis at initial inoculum density of 3.5×107 cfu/mL. Thus a differentiated application strategy for B. velezensis BMF03 was proposes: an initial inoculum density of 3.5×105 cfu/mL was used for wastewater with high nitrite and phosphate densities, 3.5×106 cfu/mL was used for wastewater contaminated with ammonia nitrogen, and 3.5×107 cfu/mL was used for wastewater with excessive total nitrogen and total phosphorus. The findings indicated the optimal application density of B. velezensis BMF03, and provided theoretical and technical support for microbial treatment technology in aquaculture wastewater.
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2026, Vol. 45 
