Comparison of Differences between Serum and Plasma Biochemical Indices inSix Common Species of Freshwater Fishes
REN Shengjie1, CHEN Wen2, XU Wenjing1, GUI Qin1, NIE Zhulan1, HUANG Zhaohua3, MA Xianjun1
1. State Key Laboratory for Conservation and Utilization of Biological Resources in Tarim Basin, College of Life Sciences and Technology, Tarim University, Alaer 843300, China; 2. Agricultural Technology ExtensionStationof Baihu Town, Hefei 231500, China; 3. Alaer Changxin Fishery Co., Ltd, Alaer 843300, China
Abstract：In order to comparatively analyze the differences in serum and plasma indicators in the blood biochemistry, sixteen routine biochemical indicators were comparatively determined between serum and plasma samples collected from caudal vein whole blood of six common species of freshwater fishes with different feeding habit including grass carp Ctenopharyngodon idellus [with body weight of (2400±177) g], silver carp Hypophthalmichthys molitrix [(2350±136) g], bighead carp Aristichthys nobilis [(1950±158) g], crucian carp Carassius auratus, [(500±36) g], yellow catfish Pelteobagrus fulvidraco [(500±71) g], and largemouth bass Micropterus salmoides [(1400±118) g] using a fully automated biochemical analyzer. The results were statistically analyzed using SPSS 22.0 software. Among all biochemical parameters with statistically significant differences between serum and plasma, the serum values were shown to be markedly higher than their plasma counterparts for every parameter except triglycerides in crucian carp and total bile acid in largemouth bass in all species. Food strongly modulated the magnitude of the serum-plasma divergence; for instance, obviously lower alkaline phosphatase activity in both serum and plasma in the filter-feeding/planktivorous silver carp than that in the other five species. These findings indicate that serum is the preferred matrix for routine clinical chemistry in freshwater teleosts, whereas anticoagulant-induced biases in plasma require species-specific correction factors to ensure analytical accuracy. The dataset provides an empirical foundation for standardizing hematological assessment in freshwater aquaculture and ecological research.
2026, Vol. 45 
