Effects of Chronic pH Stress on Physiology, Growth, and Behavior ofJuvenile Black Rockfish Sebastes schlegelii
WANG Kexiang1, YUE Yue1, LIN Shengkai1, TIAN Tao1,2,3, YU Xiaoming1,2,3, WU Zhongxin1,2,3
1. Center for Marine Ranch Engineering Technology Research of Liaoning, Dalian Ocean University, Dalian 116023, China; 2. Industry Institute of Marine Ranching, Dalian Ocean University, Dalian 116023, China; 3. Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian 116023, China
Abstract：To investigate the changes in growth,physiology and behavior of juvenile black rockfish Sebastes schlegelii under chronic pH stress, the black rockfish with body weight of (19.62±1.20) g were reared in a fiberglass tank at pH of 6.5, 7.5, 9.5, and 8.5 (control group) for 21 days. The pH of the water was gradually increased or decreased by 0.5% daily using 2 mol/L hydrochloric acid or sodium hydroxide. Results showed that significantly different growth indicators were observed in the test groups, with significantly lower weight gain rate and length-gain rate in the test groups than those in the control group (P<0.05). There were significant differences in behavioral metrics including the swimming speed, inter-individual distance, approach frequency, and distance to the edge of the observation area in the black rockfish exposed to different pH (P<0.05). Also, there were significant differences in physiological indices in the black rockfish exposed to different pH (P<0.05), with gradual increase in superoxide dismutase activity, and decrease in alkaline phosphatase, acid phosphatase, and catalase activities as pH decreased (P<0.05). Glutamatergic aminotransferase and glutathione activities were found to be first increased and then decreased under acidic conditions, and to be elevated progressively in the black rockfish exposed to alkaline conditions. In conclusion, chronic pH stress leads to significant impact on juvenile black rockfish; although the species shows some tolerance to pH fluctuations, chronic pH stress inhibits antioxidant enzyme activity, schooling, and growth and enhances group cohesion.
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2026, Vol. 45 
