Microbial Community Structure and Influencing Factors at the Bottom Mud of White Snakehead Channa argus Culture Ponds
XIONG Zhaodi1, ZHUO Ting2, FAN Wei2, JIAO Xiaolei2, XIA Feihong3, SU Jian2
1. Shanghai Vocational College of Agriculture and Forestry, Shanghai 201699, China; 2. Neijiang Academy of Agricultural Sciences, Neijiang 641000, China; 3. Neijiang Normal University, Neijiang 641100, China
Abstract：In order to understand the structural characteristics of the microbial community in the bottom mud in culture ponds of white snakehead Channa argus and relationship with the environmental factors in the bottom mud, the characteristics of the bottom mud flora of 7 white snakehead culture ponds in Dongxing District, Neijiang City, Sichuan Province were analyzed on April 28, 2023, by high-throughput sequencing technology. The associations between environment variables and the micorbial assemblage were analyzed by Person correlation analysis and redundancy (RDA). The results showed that the sediment was slightly alkaline, and the contents of total nitrogen and total phosphorus were 4.73 and 2.58 times of that in the environmental background values of Sichuan Province, respectively. A total of 2915 OTUs were obtained, covering 36 phyla, 85 classes, 185 orders, 213 families, 293 genera and 456 species; with the dominant bacterial groups Firmicutes, Proteobacteria, Actinobacteria, Chloroflexi, Acidobacterioto, Desulfobacterota and Bacteroidota. Person correlation analysis showed that Acidobacteria was significantly positively correlated with total nitrogen (P<0.05), that Proteobacteria significantly positively correlated with soil alkaline phosphatase (P<0.05), and that Actinobacteria significantly positively correlated with soil polyphenol oxidase (P<0.05). The findings will lead to further understanding of the characteristics of the microbial community and the factors affecting the environment in the healthy culture environment of white snakehead, and provide reference for the green and healthy culture of snakehead and the screening and the application of improving the bottom sediment of breeding ponds.
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2025, Vol. 44 
