Transcriptome Difference Analysis of Body Wall in Sea Cucumber Apostichopus japonicus in Response to High-Temperature Stress
SUN Hongjuan1, JIANG Jingwei1, SONG Gang2, CHEN Zhong1, GUAN Xiaoyan1, GAO Shan1, WANG Bai1, JIANG Pingzhe1, LI Peipei1, YUE Dongmei1, XIAO Yao1, PAN Yongjia1, JIANG Bing1, DONG Ying1
1. Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Liaoning Key Laboratory of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic Animals, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China; 2. Longhaixingang Science and Technology Co., Itd., Jinzhou 121209, China
Abstract：In order to explore the regulatory mechanism of juvenile sea cucumber Apostichopus japonicus in response to high temperature stress, transcriptome sequencing analysis was conducted in the body wall in the sea cucumber with body weight of (5.0±0.6) g exposed to water temperature of 30 ℃ raising from 24 ℃ at a rate of 1 ℃/h (high temperature group) and of 24 ℃ (control group). Transcriptome comparison revealed that there were 2920 differentially expressed genes (DEGs) in the body wall o fthejuvenile between the high-temperature group and the control group, including 1342 up-regulated expression genes and 1578 down-regulated expression genes. KEGG analysis showed that the DEGs were found to be significantly enriched in ribosome, protein processing in endoplasmic reticulum, pathogen interaction, glycolysis and oxidative phosphorylation pathways. The distribution of up and down regulated genes in signaling pathways showed that the signal transduction activity was increased, with decrease in the protein translation activity, increase in the folding and processing activity of existing proteins, and decrease in overall energy metabolism in the juvenile exposed to high-temperature stress. The expression levels of five genes were detected in control group and high-temperature group by quantitative real time PCR, with correlation coefficient R of 0.87, indicating that the results of transcriptome sequencing were reliable. In response to high-temperature stress, the high expression of HSP90β and HSP70A/B2 genes promoted the correct folding of protein and enhance the heat resistance, via increase in protein folding and processing. Protein translation and energy consumption were shown to be decreased, which is a strategy to maintain homeostasis and protect from injury in juvenile. Screening single nucleotide polymorphisms (SNP) on mRNAs associated with high-temperature stress is beneficial for the development of molecular markers and breeding of new heat-resistant varieties in A. japonicus.
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2025, Vol. 44 
