Effects of Chronic Ammonia Nitrogen Stress on Growth, Intestinal Digestive Enzyme Activity and Structure of Blunt Snout Bream Megalobrama amblycephala
WANG Canli, DAI Mengyang, WANG Qian, XIONG Xinyu, ZHANG Han, WANG Yifan, YE Wenhui, WANG Hengjie, YUAN Xiangyang
Biological Manufacturing Institute of Modern Food Resources, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, China
Abstract：In order to investigate the effects of chronic ammonia nitrogen stress on growth, and intestinal enzymes activity and histological structure of blunt snout bream, Megalobrama amblycephala, blunt snout bream with body weight of (6.55±0.24) g was reared in a 120 cm height × 80 cm diameter tank, and exposed to aerated tap water (control group G0), 6.82 mg/L ammonium chloride (G1) (10% 96 h LC50) and 13.64 mg/L ammonium chloride (G2) (20% 96 h LC50) at 26—27 ℃ for 4 weeks. At the end of the experiment, enzyme activity analysis, transmission micro-electron microscopy and PCR technology were conducted to analyze the intestinal enzyme activity and histological structure characteristics of the test fish. The results showed that the median lethal concentration LC50 of ammonia nitrogen solution at 96 h was 68.18 mg/L for blunt snout bream. The final body weight, survival rate, weight gain rate, specific growth rate, and activities of protease, lipase, amylase, Na+/K+-ATPase, and γ-GT were shown to be significantly higher in the fish in G0 group than those in G1 and G2 groups (P<0.05). There was significantly higher intestinal microvillus length in blunt snout bream in G0 than those in G1 and G2 groups (P<0.05). Chronic ammonia nitrogen stress led to down-regulated relative expression levels of Claudin-3c, Claudin-7, Occludin and ZO-1 genes compared to those in G0 group, and to up-regulated relative expression levels of TNF-a, IL-1B and IL-6 (P<0.05). In conclusion, chronic ammonia nitrogen stress inhibited the growth, and decreased digestive and absorptive function of blunt snout bream.
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