Mechanistic Analysis of Tannic Acid in Treatment of Parasite Ichthyophthirius multifiliis by Quantitative Proteomics
CHENG Xu1, SUN Gan2, QU Huantao1, GUO Wentao1, LI Maohua1, CHEN Pei1, ZHAO Yu1, ZHU Jian1, YANG Jing1
1. Hubei Key Laboratory of Three Gorges Project for Fish Resource Conservation, Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang 443100, China; 2. China Three Gorges Construction Engineering Corporation, Chengdu 610041, China
Abstract：To explore the efficacy of tannic acid in killing fish parasite Ichthyophthirius multifiliis and its potential mechanisms of action, the morphological changes of the protomonts, tomont, and cyst stages of I. multifiliis were analyzed after treatment with tannic acid at concentrations of 15, 20, and 25 mg/L at 23 °C, along with the effects of the drug in vitro pharmacodynamic evaluation and quantitative proteomics. The results showed that membrane disintegration in protomonts, developmental arrest of tomonts, and complete mortality of theronts were observed in I. multifiliis exposed to 25 mg/L tannic acid for 10 minutes. The results of quantitative proteomic analysis showed that 98 proteins were significantly upregulated and 125 proteins were significantly downregulated in I. multifiliis in the treatment group (EG: 25 mg/L, 10 min) than that in the control group (CG: no drug treatment). GO enrichment analysis revealed that the differentially expressed proteins (DEPs) were mainly associated with functions related to cell metabolism, cell adhesion, and cell structure. The KEGG pathway enrichment analysis revealed that the ribosome pathway was the most significantly enriched for DEPs. In this study, a total of 16 DEPs were found to be significantly downregulated in the ribosome pathway, of which 12 were ribosomal proteins. These findings demonstrate that the ribosomal pathway constitutes the most significantly perturbed functional axis in I. multifiliis following tannic acid exposure, which provides theoretical basis for comprehensive elucidation of the treatment mechanism of tannic acid in I. multifiliis killing.
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2026, Vol. 45 
