|
|
|
| Effect of Post-Harvested Air Exposure on Quality of Three Species of Molluscs |
| YAN Lixin, JIANG Minghui, LIU Junrong, YI Hongli, WANG Xuanfei, TIAN Yuanyong |
| College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China |
|
|
|
|
Abstract In order to study the air exposure tolerance of cultured economically important molluscs in early post-harvest process, vigorous Manila clam Ruditapes philippinarum, Pacific oyster Crassostrea gigas and Yesso scallop Patinopecten yessoensis were selected to put in the air exposure condition for 8 d in 4 ℃. The contents of glycogen, ATP related compounds and arginine phosphate, pH, and opine dehydrogenase activity in adductor muscle were detected to study the quality change in this process. The results showed that during the period of air-exposure storage, there was no significant change in the pH value of the molluscs (P<0.05), while the pH values were decreased from 7.23 and 7.39 to 6.88 and 6.80 in oyster and scallop, respectively. The glycogen content of the three species of molluscs showed a decreasing trend. The initial contents of ATP in clam and oyster were only 0.41 and 0.42 μmol/g, with no significant change during storage (P<0.05), while the initial content of ATP in scallop was 4.05 μmol/g, and decreased sharply with the extension of storage time. The activity of ODH was the highest in the adductor muscle of scallop and clam, and showed an increasing trend with the storage time, while ADH and SDH played bigger roles in oyster. Combined with principal component analysis, it was concluded that the tolerance time of oyster, clam and scallop in air exposure was 8 d, 4 d and 2 d, respectively. The findings indicated that the establishment of specific management measures should be combined with the difference of air exposure tolerance in the circulation process of post-harvested molluscs.
|
|
Received: 28 October 2019
|
|
|
|
|
|
[1]农业部渔业渔政管理局. 中国渔业统计年鉴2018[M].北京:中国农业出版社,2018.
[2]刘金洋. 虾夷扇贝在活品流通过程中的代谢变化[D].大连:大连海洋大学,2016.
[3]刘慧慧. 采捕后菲律宾蛤仔的代谢规律与品质评价[D].大连:大连海洋大学,2018.
[4]刘慧慧,周晏琳,张晴,等. 菲律宾蛤仔捕后干露处置对其复水湿藏稳定性的影响[J]. 大连海洋大学学报,2018,33(2):244-250.
[5]Kawabe S, Takada M, Shibuya R, et al. Biochemical changes in oyster tissues and hemolymph during long-term air exposure[J].Fisheries Science,2010,76(5):841-855.
[6]Harcet M, Perina D, Plee B. Opine dehydrogenases in marine invertebrates[J].Biochemical Genetics,2013,51(9/10):666-676.
[7]Livingstone D R, de Zwaan A, Leopold M, et al. Studies on the phylogenetic distribution of pyruvate oxidoreductases[J].Biochemical Systematics and Ecology,1983,11(4):415-425.
[8]Sato M, Takeuchi M, Kanno N, et al. Distribution of opine dehydrogenases and lactate dehydrogenase activities in marine animals[J].Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,1993,106(4):955-960.
[9]刘慧慧,刘俊荣,田元勇,等. 易逝期干露对菲律宾蛤仔活品净化及贮藏特性的影响[J]. 大连海洋大学学报,2019,34(1):112-121.
[10]Matsumoto M, Yamanaka H. Studies on rigor-mortis of kuruma prawn muscle[J]. Nihon-suisan-gakkai-shi,1991,57(11):2121-2126.
[11]Gornall A G, Bardawill C J, David M M. Determination of serum proteins by means of the biuret reaction[J].The Journal of Biological Chemistry,1949,177(2):751-766.
[12]Bailey D M, Johnstone I A. Scallop swimming kinematics and muscle performance: modelling the effects of “within-animal” variation in temperature sensitivity[J]. Marine & Freshwater Behaviour & Physiology,2005,38(1):1-19.
[13]李亚烜,闫丽新,于笛,等. 不同规格虾夷扇贝捕后耐干露特性比较[J]. 水产科学,2019,38(4):443-450.
[14]Kimura M. Studies on the quality control of the Japanese scallop adductor muscle[J]. Entific Reports of Hokkaido Fisheries Experimental Station,2003,65:1-47.
[15]Livera W C D, Shimizu C. Comparison and characterization of arginine kinases purified from the prawn Penaeus japonicus (Kurumaebi) and the swimming crab Portunus trituberculatus (Gazami)[J]. Journal of the Agricultural Chemical Society of Japan,2014,53(9):2377-2386.
[16]Lee A, Lee K. Studies on the activities of OPINE and lactate dehydrogenase in gill, mantle, foot and adductor muscle of hard clam[J]. Journal of Marine Science and Technology,2011,19(4):361-367.
[17]Vollmer M, Hochachka P W, Mommsen T P. Octopine dehydrogenase and phosphoarginine kinase in squid mantle: cooperation of two enzymes at the arginine branchpoint in cephalopod muscle[J]. Canadian Journal of Zoology,1981,59(7):1447-1453.
[18]Storey K B. Purification and properties of adductor muscle phosphofructokinase from the oyster, Crassostrea virginica.The aerobic/anaerobic transition:role of arginine phosphate in enzyme control[J].European Journal of Biochemistry,1976,70(2):331-337.
[19]郑尧. 虾夷扇贝闭壳肌中的章鱼碱脱氢酶及其捕后变化[D].大连:大连海洋大学,2018.
[20]DeZwaan A, Putzer V. Metabolic adaptations of intertidal invertebrates to environmental hypoxia (a comparison of environmental anoxia to exercise anoxia)[J].Symposia of the Society for Experimental Biology,1985,39:33-62.
[21]Hochachka P W. Fuels and pathways as designed systems for support of muscle work[J].The Journal of Experimental Biology,1985,115:149-164.
[22]Fields J H A, Storey K B. Tissue-specific alanopine dehydrogenase from the gill and strombine dehydrogenase from the foot muscle of the cherrystone clam Mercenaria mercenaria (Linn.) [J]. Journal of Experimental Marine Biology and Ecology,1987,105(2/3):175-185.
[23]Nakanishi K, Fujii T, Szekely J. Possible relationship between energy dissipation and agitation in steel processing operations[J]. Ironmaking & Steelmaking,1975,2(3):193-197.
[24]Gde G. Purification and properties of tauropine dehydrogenase from the shell adductor muscle of the ormer, Haliotis lamellosa[J].European Journal of Biochemistry,1986,160(2):311-318.
[25]Grieshaber M K, Hardewig I, Kreutzer U, et al. Physiological and metabolic responses to hypoxia in invertebrates[J]. Reviews of Physiology Biochemistry & Pharmacology,1994,125(125):43-147.
[26]刘洋,李亚烜,徐美禄,等. 糖代谢关键酶活性指示的虾夷扇贝活品品质变化[J]. 大连海洋大学学报,2019,34(5):704-709.
[27]Carvajal N, Kessi E. Properties of octopine dehydrogenase from the foot muscle of Concholepas concholepas[J]. Comparative Biochemistry and Physiology—Part B: Biochemistry and Molecular Biology,1988,90(1):77-79.
[28]Storey K B. Tissue-specific alanopine dehydrogenase and strombine dehydrogenase from the sea mouse, Aphrodite aculeata (polychaeta)[J]. Journal of Experimental Zoology,2010,225(3):369-378.
[29]Vidode Mattio N D, Paredi M E, Crupkin M. Postmortem changes in the adductor muscle of scallop (Chlamys tehuelchus) in chilled and frozen storage[J]. Journal of Aquatic Food Product Technology,2001,10(3):49-60. |
|
|
|
