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| Effects of Ultraviolet Radiation Intensity on Embryonic Development of Chinese Freshwater Prawn Neocaridina denticulata sinensis |
| SONG Ziyu, WANG Guodong, ZHANG Lili, HUANG Shiyu, HONG Wei, JIA Ben, ZHANG Yuanchang |
| College of Fisheries, Jimei University, Xiamen 361021, China |
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Abstract The embryos of Chinese freshwater prawn Neocaridina denticulata sinensis were detached in petri dishes from the abdomen at stages of cleavage, blastula, gastrula and nauplius larvaⅠand exposed to ultraviolet radiation (UV) at doses of 0 W·s/cm2, 1 W·s/cm2, 2 W·s/cm2, 4 W·s/cm2, 8 W·s/cm2 and 16 W·s/cm2 in a manual shaking incubator at a speed of 55 r/min, a temperature of 25 ℃, and a humidity of 60%. The embryonic development was observed under a dissecting scope every 24 hours, and data analysis was performed after 48 h to investigate the effect of UV dose on the embryonic development of Chinese freshwater prawn. The results showed that Chinese freshwater prawn had the survival rate of the embryo in a dose-dependent relationship with UV dose exposure with severely delayed embryo developmen with the increase in UV dose. The median lethal dose at the 48 h was found to be 11.5 W·s/cm2 in cleavage stage, 8.6 W·s/cm2 in blastula stage, 12.0 W·s/cm2 in gastrula stage, and 14.2 W·s/cm2 in nauplius I stage. In addition, exposure period showed significant impact on embryonic development.
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Received: 18 November 2019
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[1]林师,王国栋,张丽莉,等.观赏中华锯齿米虾的基础生物学和繁养技术[J].科学养鱼,2016(7):80-81.
[2]Charron R A, Fenwick J C, Lean D R, et al. Ultraviolet-B radiation effects on antioxidant status and survival in the zebrafish, Brachydanio rerio[J].Photochemistry and Photobiology,2000,72(3):327-333.
[3]Sayed A E H, Mitani H. The notochord curvature in medaka (Oryzias latipes) embryos as a response to ultraviolet A irradiation[J].Journal of Photochemistry and Photobiology B:Biology,2016,164:132-140.
[4]Volz D C, Wirth E F, Fulton M H, et al. Endocrine-mediated effects of UV-A irradiation on grass shrimp (Palaemonetes pugio) reproduction[J].Comparative Biochemistry and Physiology.Toxicology & Pharmacology,2002,133(3):419-434.
[5]庄惠如,陈必链,陈荣,等.雨生红球藻的紫外、激光复合诱变育种[J].激光生物学报,2001,10(2):135-139.
[6]张敏,李春阳,王克玉,等.不同中波紫外线对豚鼠皮肤色素沉着及α-黑素细胞刺激素表达的影响[J].山东大学学报(医学版),2011,49(3):59-63.
[7]Yan X H. Studies on color type variants from mutagenized protoplasts of Porphyra haitanensis Chang et Zheng & P. yezoensis Ueda (Rhodophycease)[J].Chinese Journal of Oceanology and Limnology,1993,11(3):235-244.
[8]薛俊增,郑璐,陈樟福.中华新米虾胚胎发育的初步研究[J].杭州师范学院学报,1995,17(6):79-83.
[9]谢军龙,孟伟,沈国民.中央空调中消毒紫外线灯强度场计算方法[J].制冷与空调,2004,4(5):63-65.
[10]Lud D, Moerdijk T C W, van de Poll W H, et al. DNA damage and photosynthesis in Antarctic and Arctic Sanionia uncinata (Hedw.) Loeske under ambient and enhanced levels of UV-B radiation[J].Plant, Cell and Environment,2002,25(12):1579-1589.
[11]严正凛,关瑞章,胡家财,等.紫外线照射剂量对九孔鲍胚胎及幼虫的影响[J].台湾海峡,2007,26(3):404-409.
[12]Amemiya S, Yonemura S, Kinoshita S, et al. Biphasic stage sensitivity to UV suppression of gastrulation in sea urchin embryos[J].Cell Differentiation,1986,18(1):45-49.
[13]鄂晓飞,朱旭东,李晶,等.不同抗氧化剂在紫外线所致人体皮肤环丁烷嘧啶二聚体形成中的作用[J].癌变·畸变·突变,1998,10(6):331-335.
[14]刘俊平,安志兴,张涌.紫外线照射时间对孤雌激活和体外受精胚胎发育的影响[J].动物医学进展,2004,25(2):109-110,125.
[15]Rupp W D. Early days of DNA repair:discovery of nucleotide excision repair and homology-dependent recombinational repair[J].The Yale Journal of Biology and Medicine,2013,86(4):499-505.
[16]Lindahl T. My journey to DNA repair[J].Genomics,Proteomics & Bioinformatics,2013,11(1):2-7. |
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