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| Eye Development-Related Genes Involved in Embryonic Development Period of Japanese Spineless Cuttlefish Sepiella japonica |
| LI Shaogang1, LI Yongqin1,2, XU Lele1,2, CHEN Daohai1,2, WANG Liyun1,2,3 |
1. College of Science and Technology, Lingnan Normal University, Zhanjiang 524048, China;
2. Engineering ResearchCenter of Seafood Resource Sustainable Ultilization of the West of Guangdong Province, Zhanjiang 524048, China;
3. Guangdong Provincial Key Laboratory of Development and Education for Special Needs Children, Zhanjiang 524048, China |
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Abstract Genes of SIX Homeonox 3 (six3), Eyes Absent (eya), and Paired Box 6 (pax6) involved in the eye development were selected from the transcriptome database established from Japanese spineless cuttlefish Sepiella japonica embryonic stage by Illumina HiSeqTM 4000 high-throughput sequencing. The relative transcriptive expression level of these three genes in three embryo periods (eye primordium formation period, functional organ differentiation period, and larval period), and eight stages of Japanese spineless cuttlefish were detected by the RT real-time PCR. The results showed that the expression level of eye development-related genes of Japanese spineless cuttlefish was different during the three embryonic development periods. The relative transcriptive level of six3 and eya genes was increased significantly from the eye primordium formation period to the larval period. However, the relative transcriptive level of pax6 gene was increased from the eye primordium formation period to the functional organ differentiation period, and subsequently decreased gradually till to the larval period. It is proposed that six3, eya, and pax6 genes play different regulatory functions in the retinal determination gene network network, and have important significance for regulation of the eye development in embryo of Japanese spineless cuttlefish.
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Received: 27 March 2020
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