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| Microbial Diversity of Skin and Liver of Diseased Grass Carp Evaluated by High-Throughput Sequencing of 16S rRNA Gene |
| SUN Kun1, WANG Xiaolei2, ZHANG Jie1, FU Longwei3, LIU Xuemei4, ZHANG Yanzhen3, SUI Zhihai1, LIU Yunguo1 |
1. College of Life Sciences, Linyi University, Linyi 276000, China;
2. College of Education, Linyi University, Linyi 276000, China;
3. College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China;
4. Agricultural Technology Extension Station of Hedong District, Linyi 276003, China |
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Abstract Grass carp Ctenopharyngodon idella is an important freshwater aquaculture species, but infection diseases induced by the bacterial pathogens cause serious economic losses. We used high-throughput sequencing of the V4 region of 16S rRNA gene to quickly evaluate the bacterial communities of skin and liver of diseased grass carp from different ponds. The results showed that the number of OTUs in the skin and liver of grass carp was 611 and 875 in pond No.1, 376 and 644 in pond No.2, more in pond No.1 than that in pond No. 2. Alpha diversity analysis revealed that the abundance and diversity of bacteria of the liver from pond No.1 were the highest. The beta diversity analysis indicated that there were similar community structures in the skin of No.1, and the skin and liver of No.2, but the bacterial community structure of liver from pond No.1 was different from the other three samples, indicating that the infection status of grass carp in the pond No.1 was more complicated than that in pond No.2. In the bacterial community structure of the all samples, we found that the dominant phylum in the phylum classification level was mainly Bacteroidetes and Proeobacteria. The dominant genera in the genus classification level were mainly Flavobacterium and Shewanella, which was most likely to be the dominant pathogenic bacteria of this disease. The findings could provide the scientific basis for disease prevention and control in early stage, and also provide guidance to the isolation and identification of the bacteial pathogens in later stage.
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Received: 02 July 2020
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