Cu<sup>2+</sup>对文蛤红壳色选育系及自然群体抗氧化能力的影响

doi:10.16378/j.cnki.1003-1111.20145

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Abstract Cu²⁺ as one of the main pollutants of heavy metal pollution in the ocean is characterized by wide source, easy residue, and easy enrichment of food chain, which constitutes a potential ecological crisis and economic crisis for the cultivation of marine shellfish. In order to understand the tolerance of natural populations Meretrix meretrix and red shell color breeding lines of hard cham to different concentrations of Cu²⁺, the effects of different concentrations of Cu²⁺ on the activities of superoxide dismutase (SOD) and catalase (CAT) and the relative expression of Cu/Zn-SOD gene in hard clam were studied. The results showed: (1) at the same concentration of Cu²⁺, the survival rate of red shell color breeding line hard clam was found to be slightly higher than that of natural population, without significant difference (P>0.05). (2) The changes in SOD and CAT enzyme activities of hard clam under different concentrations of Cu²⁺ showed the corresponding time effect relationship and concentration effect relationship, and the SOD enzyme activity of hard clam showed a linear upward trend, while CAT activity showed a first trend, with significant difference between red shell color breeding line and natural population (P<0.05). (3) The expression of Cu/Zn-SOD gene was consistent with the expression of SOD enzyme activity. With the linear expression of Cu²⁺ concentration, the maximal relative expression of gene was observed at Cu²⁺ concentration of 0.4 mg/L. (4) The antioxidant activity of red shell color breeding line hard clam was better than that of natural population. The purpose of this study is to lay a theoretical foundation for the selection of stress resistance and the development of new species of hard clam.

Key words： Cu²⁺ Meretrix meretrix red shell color breeding line SOD CAT

Received: 07 July 2020

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	TIAN Zhen
	ZHANG Zhidong
	CHEN Aihua
	WU Yangping
	CHEN Suhua
	ZHANG Yu
	CAO Yi
	LI Qiujie

Cite this article:

TIAN Zhen,ZHANG Zhidong,CHEN Aihua, et al. Effect of Cu²⁺ on Antioxidant Capacity of Red Shell Color Breeding Line and Natural Population of Hard Clam Meretrix meretrix[J]. Fisheries Science, 2022, 41(1): 62-68.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20145 OR https://www.shchkx.com/EN/Y2022/V41/I1/62

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