克氏原螯虾烂尾病病原的分离鉴定及其相关特性分析

doi:10.16378/j.cnki.1003-1111.20151

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Abstract The purpose of this experiment was to study the species and related characteristics of pathogen which caused tail-rotted disease of red swamp crayfish Procambarus clarkii. Two representative bacterial strains with different colony morphologies and colors were isolated from infectious location of diseased red swamp crayfish, and known as LW2047-1 and LW2047-2. The pathogen bacteria were confirmed as strain LW2047-2 by artificial infection test. We observed strain LW2047-2 with bacteriological methods and analyzed that by morphological, physiological and biochemical characteristics. Finally, strain LW2047-2 was identified as Citrobacter freundii when 16S rRNA and gyrB gene were sequenced and analyzed. CFA gene and chi gene were detected for analysis of adhesion and chitin decomposition characteristics. Results of antimicrobial sensitivity test showed that strain LW2047-2 was sensitive to enrofloxacin and neomycin sulfate and insensitive to sulfa drugs.

Key words： Procambarus clarkii tail-rotted disease Citrobacter freundii isolation and identification characteristics analysis

Received: 13 July 2020

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	Articles by authors
	LIU Zhanghuai
	WU Ting
	WANG Jiajun
	ZHU Chunyan
	ZHANG Yingying
	MENG Qingguo

Cite this article:

LIU Zhanghuai,WU Ting,WANG Jiajun, et al. Isolation, Identification and Related Characteristics Analysis of Pathogen Isolated from Red Swamp Crayfish Procambarus clarkii with Tail-Rotted Disease[J]. Fisheries Science, 2022, 41(1): 102-109.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20151 OR https://www.shchkx.com/EN/Y2022/V41/I1/102

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