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| Growth and Single Nucleotide Polymorphism Loci Analysis of Myostatin Gene in ENU Mutagenesis Grass Carp Family |
| WANG Chenglong, CHEN Jie, JIANG Xiayun, ZOU Shuming |
| Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Ministry of Agricultureand Rural Affairs, Shanghai Ocean University, Shanghai 201306, China |
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Abstract Juveniles of mutagenesis grass carp (Ctenopharyngodon idellus) by N-ethyl-N-nitrosourea (ENU) in four families were reared in a 6 m × 4 m × 1.5 m cement pond for 175 days to compare the growth. Significant comparisons, partial correlation analysis, and factor analysis statistical methods were used to analyze body weight, total length, body length, head length, body depth, caudal peduncle length, caudal peduncle height, and body width and single nucleotide polymorphism (SNP) loci of myostatin (MSTN) gene MSTN1 and MSTN2 genes in families with significant differences screened by two-way sequencing. There was significantly better growth in family 4 than that in family 3, with significantly higher 8 characters in family 4 than in the other families (P<0.05). The partial correlation analysis of the relationship between the morphological traits and body weight indicated that there were correlation coefficient of 0.357 between total length and body weight, 0.619 between body length and body weight, 0.608 between caudal peduncle length and body weight, and 0.396 between body width and body weight in family 1; there were correlation coefficient of 0.348 between total length and body weight, 0.360 between body length and body weight, 0.687 between head length and body weight, and -0.384 between body width and body weight in family 2; in family 3 there were correlation coefficient of 0.529 total length, 0.449 body length, -0.351 caudal peduncle length, and 0.384 caudal peduncle height; and in family 4 there were 0.629 total length, 0.543 body length, and 0.590 body width, showing the significat difference in growth between family 4 and family 3. For MSTN1, 465 nt C/G and 467 nt G/A in family 3, and 465 nt C/G in family 4 were found missense mutations; For MSTN2, 912 nt C/T in family 3 and family 4 was found synonymous mutation, 1027 nt G/A in family 3,and 366 nt A/G and in family 4 were found missense mutations, and SNP sites were found in both noncoding regions 1390 nt A/T and 1401 nt G/A in both families. The findings indicate that the SNPs of MSTN1 and MSTN2 genes are closely related to the growth traits of ENU mutagenesis grass carp.
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Received: 30 January 2018
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[1]Mullins M C, Hammerschmidt M, Haffter P, et al. Large scale mutagenesis in the zebrafish:in search of genes controlling development in a vertebrate[J]. Curr Biol,1994,4(3):189-202.
[2]Van Eeden F J, Granato M, Odenthal J, et al. Developmental mutant screens in the zebrafish[J]. Meth Cell Biol,1999,60:21-41.
[3]Hitotsumachi S, Carpenter D A, Russell W L. Dose-repetition increases the mutagenic effectiveness of N-ethyl-N-nitrosourea in mouse spermatogonia[J]. Proc Natl Acad Sci USA,1985,82(19):6619-6621.
[4]Driever W, Solnica-Krezel L, Schier A F, et al. A genetic screen for mutations affects embryogenesis in zebrafish[J]. Development,1996,123:37-46.
[5]Jin P, Tian T, Sun Z, et al. Generation of mutants with developmental defects in zebrafish by ENU mutagenesis[J].Chinese Sci Bull,2004,49(20):2154-2158.
[6]韩启霞,常玉梅,梁利群,等.ENU诱变银鲫F1的遗传差异分析[J].中国水产科学,2010,17(5):895-902.
[7]Jiang X Y, Sun C F, Zou S M, et al. Enu-induced mutagenesis in grass carp (Ctenopharyngodon idellus) by treating mature sperm[J]. PLoS One,2011,10(6):e26475.
[8]Mccroskery S, Thomas M, Platt L, et al. Improved muscle healing through enhanced regeneration and reduced fibrosis in myostatin-null mice[J]. Journal of Cell Science,2005,118(15):3531-3541.
[9]Marcq F, Barkouki S E I, Elsen J M, et al. Investigating the role of myostatin in the determinism of double muscling characterizing Belgian Texel sheep[J]. Animal Genetics,1998,29(Suppl.1):52.
[10]薛良义,李婷,孙升,等.大黄鱼肌肉生长抑制素基因外显子I遗传多态性分析[J].水产科学,2008,27(10):503-506.
[11]Dunner S, Miranda M E, Amigues Y, et al. Haplotype diversity of the myostatin gene among beef cattle breeds [J]. Genetics Selection Evolution,2003,35:103-118.
[12]Langley B, Thomas M, Bishop A, et al. Myostatin inhibits myoblast differentiation by down-regulating MyoD expression[J]. The Journal of Biological Chemistry,2002,277(51):49831-49840.
[13]Lee S J, Lee Y S, Zimmers T A, et al. Regulation of muscle mass by follistatin and activins [J]. Molecular Endocrinology,2010,24(10):1998-2008.
[14]Lee S J, Mcpherron A C. Regulation of myostatin activity and muscle growth[J].Proceedings of the National Acad Sciences,2001,98(16):9306-9311.
[15]Xu C, Wu G, Zohar Y, et al. Analysis of myostatin gene structure, expression and function in zebrafish [J]. The Journal of Experimental Biology,2003,206(Pt 22):4067-4079.
[16]Bauer H, Meier A, Hild M, et al. Follistatin and noggin are excluded from the zebrafish organizer [J]. Developmental Biology,1998,204(2):488-507.
[17]Rescan P Y, Jutel I, Ralliere C. Two myostatin genes are differentially expressed in myotomal muscles of the trout (Oncorhynchus mykiss)[J]. The Journal of Experimental Biology,2001,204(Pt 20):3523-3529.
[18]Medeiros E F, Phelps M P, Fuentes F D, et al. Overexpression of follistatin in trout stimulates increased muscling [J]. American Journal of Physiology,2009,297(1):R235-R242.
[19]Gottesman M M, Fojo T, Bates S E. Multidrug resistance in cancer:role of ATP-dependent transporters[J]. Nat Rev Cancer,2002,2(1):48-58.
[20]濮剑威,孙成飞,蒋霞云,等.草鱼两个肌肉生长抑制素cDNA克隆、表达及过量表达对胚胎发育的影响[J].生物技术通报,2011,8(19):153-166.
[21]丁馥香,张跟喜,王金玉,等.边鸡胰岛素样生长因子1基因(IGF-1)外显子3的多态性及其与繁殖性能的关系[J].农业生物技术学报,2010,18(2):313-317.
[22]张跟喜,丁馥香,是燕萍,等.肌肉生长抑制基因(MSTN)外显子1的多态性及其边鸡生长性状的关联分析[J].农业生物技术学报,2011,19(1):122-127.
[23]刘晓琴,马喜山,唐中林,等.猪MSTN基因的多态性和生长性状关联分析[J].畜牧兽医学报,2013,44(7):1063-1069.
[24]闵令江,丰艳妮,李兰,等.山羊MSTN基因多态性与主要经济性状的关联分析[J].畜牧兽医学报,2015,46(9):1515-1524.
[25]梁春年,阎萍,刑成峰,等.牦牛MSTN基因内含子2多态性及生长性状的相关性[J].华中农业大学学报:自然科学版,2011,30(3):285-289.
[26]朱媛媛,梁宏伟,李忠,等.黄颡鱼MSTN基因多态性及其与生长性状的相关性分析[J].遗传,2012,34(1):72-78.
[27]俞菊华,李红霞,唐永凯,等.建鲤生长抑制素基因(MSTN)的分离、表达及多态性与体型、平均日增重相关性研究[J].农业生物技术学报,2010,18(6):1062-1072.
[28]永凯,李建林,俞菊华,等.吉富罗非鱼MSTN基因结构及其多态性与生长性状的相关性[J].中国水产科学,2010,17(1):44-51.
[29]张猛,陈勇,沈玉帮,等.草鱼MSTN-1基因多态性及与早期生长性状和肌肉成分关联分析[J].水产学报,2016,40(4):618-625.
[30]佟雪红,董在杰,缪为民,等.建鲤与黄河鲤的杂交优势研究及主要生长性状的通径分析[J].大连水产学院学报,2007,22(3):159-163.
[31]李思发,李家乐,李晨虹,等.尼罗罗非鱼五品系生长性能评估[J].水产学报,1998,22(4):314-321.
[32]庆宁,吕凤义,赵俊,等.华南沿海地区西部入海水系中间黄颡鱼的形态变异及地理分化[J].动物学研究,2007,28(2):207-212. |
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