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| Microsatellite Screening and Genetic Diversity Analysis of Gudgeon Pseudohemiculter dispar in Pearl River Basin |
| SU Yuling1, LI Min2, YANG Yongchun1, LIU Jiahao1, LI Zhenhai1, RUAN Huiting1, DAI Jiage1, LIU Li1, ZOU Keshu1 |
1.College of Marine Science, South China Agricultural University, Guangzhou 510642, China;
2.Key Laboratoryof Open-Sea Fishery Development, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries ResearchInstitute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China |
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Abstract Thirty-six polymorphic microsatellite markers were screened in gudgeon Pseudohemiculter dispar (Peters 1880), a small economical wild fish distributed in various freshwater drainages of south Yangtze River, by RAD-Seq technology, including 29 high polymorphism (polymorphic information content, PIC>0.50), 6 moderate polymorphism (0.25<PIC<0.50), and 1 low polymorphism (PIC<0.25). Six highly polymorphic microsatellite loci were used to analyze the genetic diversity and genetic differentiation of 8 populations of gudgeon in Pearl River basin including Beijiang, Guijiang, Duliujiang, Rongjiang, Zuojiang, Youjiang, Qianjiang and Yujiang, with alleles of 98.17, observing allele number of 12.2713, effective allele number of 6.0575, observed heterozygosity of 0.5389, expected heterozygosity of 0.7679, Shannon′s Information Index of 1.8974 and PIC of 0.7379, indicating that there was high level of genetic diversity among 8 populations of gudgeon in Peal River basin. The analysis of molecular variance showed that average populations differentiation (Fst) was 0.1028 and the inbreeding coefficient (Fis) was 0.2987, which implied that 10.28% genetic variation was between populations and 89.72% variation within population, showing the moderate level of genetic differentiation. The gene flow varying from 1.0075 to 4.7308 suggested that 8 populations be stable enough to resist genetic drift. The findings provided scientific basis for genetic resource preservation and management in gudgeon.
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Received: 28 January 2019
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