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| In Vitro Antibacterial Effect of Combination of Antimicrobial Agents against Pathogen Shewanella algae |
| CAO Haipeng1,2,3, YANG Lijun1,4, CHEN Sijia1,4, GUO Cheng1,4, YANG Xianle1,4 |
1.National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China;
2.Shanghai Engineering Research Center for Aquaculture, Shanghai 201306, China;
3.Shanghai Collaborative Innovationfor Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China;
4.National DemonstrationCenter for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China |
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Abstract The in vitro antibacterial activity of gentamicin sulfate and cefotaxime sodium against pathogenic Shewanella alage SFH3 isolated from Pacific white leg shrimp Litopenaeus vannamei with black spot disease was assayed in comparison with the six fishery antimicrobials, then the in vitro combined inhibitory effect of gentamicin sulfate and cefotaxime sodium was determined at different combined proportions by the tube double broth dilution method, and the in vitro combined bactericidal effect was further confirmed by time-kill curve assay in order to assess the potential of gentamicin sulfate and cefotaxime sodium in combination against aquatic pathogen S. alage SFH3. It was found that minimum inhibitory concentration (MIC) was 80 mg/L in gentamycin sulfate and 160 mg/L in cefotaxime sodium against the pathogen SFH3, significantly lower than thiamphenicol, doxycycline, oxolinic acid, sodium sulfamonomethoxine, sulfamethoxazole, and enrofloxacin hydrochloride. The combination of gentamicin sulfate and cefotaxime sodium showed synergistic inhibitory activities against S. alage SFH3 at combined proportions of 8∶2, and 7∶3 with MICs of 40 mg/L. In addition, 40 mg/L gentamicin sulfate and 80 mg/L cefotaxime sodium had synergistic bactericidal effect against the pathogen SFH3. This finding confirms that the combination of gentamicin sulfate and cefotaxime sodium can contribute to the in vitro antibacterial activity, which provides scientific reference with the drug control of aquatic pathogen S. alage.
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Received: 07 November 2018
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[1]吕爱军,穆阿丽,徐宏伟,等. 抗生素在水产养殖中的应用[J]. 中国动物保健,2006(12):42-44.
[2]郑宝英,张杰. 抗生素的联合用药[J]. 中国抗生素杂志,2007,32(6):324-328.
[3]莫剑良. 浅谈抗生素联合用药之利弊[J]. 科技创新导报,2010(32):243-243.
[4]Gressier M, Mbayo D, Deramond H, et al. First case of human spondylodiscitis due to Shewanella algae[J]. International Journal of Infectious Diseases, 2010,14(9):261-264.
[5]崔海荣,公衍文,索淑会. 白血病患者海藻希瓦菌血液感染1例[J]. 实用医药杂志,2017,34(10):873-874.
[6]陈偿,胡超群,陈晓燕,等. 新发现的红拟石首鱼溃疡病病原海藻施万氏菌的分离和分子鉴定[J]. 海洋与湖沼,2003,34(1):1-8.
[7]张继挺,朱文渊,王国良. 美国红鱼肾肿大症的病原及其致病性研究[J]. 宁波大学学报:理工版,2013,26(1):6-11.
[8]Li S, Zhang J, Qiu D, et al. Biological characteristics and pathogenicities of Shewanella algae and Shewanella abalone from Babylonia[J]. Agricultural Science & Technology,2015,16(9):1845-1850,1859.
[9]Cao H P, Chen S J, Lu L Q, et al. Shewanella algae: an emerging pathogen of black spot disease in freshwater-cultured whiteleg shrimp Penaeus vannamei[J]. Israeli Journal of Aquaculture-Bamidgeh,2018,70(1):1-7.
[10]中华人民共和国农业部. 中华人民共和国农业部公告第235号[EB/OL]. (2002-12-24)[2019-03-23]. http:∥jiuban.moa.gov.cn/zwllm/nybz/200803/t20080304_1028649.htm.
[11]中华人民共和国农业部. 中华人民共和国农业部公告第1997号[EB/OL]. (2013-09-30)[2019-03-23]. http:∥www.moa.gov.cn/govpublic/SYJ/201310/t20131010_3625542.htm.
[12]李焯新,蔡小辉,黄瑜,等. 中草药与抗生素联用对罗非鱼源无乳链球菌的体外抑菌作用[J]. 广东海洋大学学报,2016,36(4):45-49.
[13]邬苏晓,肖正中,李淑仪. 20种中草药水提物的体外抑菌实验[J]. 安徽农业科学,2008,36(19):8104-8105.
[14]凌空,丁诗华,吴兴镇,等.中草药体外抑杀大鲵致病性豚鼠气单胞菌的药效研究[J]. 水产科学,2015,34(7):405-412.
[15]周德庆. 微生物学教程[M]. 北京:高等教育出版社,2011:176.
[16]刘唤明,张文滔,吴燕燕,等. 脂肽和茶多酚对副溶血弧菌的协同抑菌效应和机理[J]. 食品科学,2017,38(13):14-19.
[17]宫霞,乐国伟,李云飞. 家蝇幼虫抗菌肽的抗菌谱及其与抗生素的协同作用研究[J]. 微生物学报,2005,45(4):516-520.
[18]Dong J, Ruan J, Xu N, et al. In vitro synergistic effects of fisetin and norfloxacin against aquatic isolates of Serratia marcescens[J]. FEMS Microbiology Letters,2016,363(1):1-5.
[19]An J, Zuo G Y, Hao X Y, et al. Antibacterial and synergy of a flavanonol rhamnoside with antibacterial drugs against clinical isolates of methicilin-resistant Staphylococcus aureus (MRSA)[J]. Phytomedicine,2011,18(11):990-993.
[20]赵黎芳,成玉萍,刘丽军,等.上海市闵行区副溶血性弧菌污染水产品及引起食源性疾病的流行病学分析[J]. 中国初级卫生保健,2014,28(3):57-59,62.
[21]卢静,王振宁,陈锐,等. 几种中药单体和抗生素对嗜水气单胞菌及温和气单胞菌的体外抑菌活性研究[J]. 水生生物学报,2013,37(6):1128-1132.
[22]马弋,朱必婷,王田. 大蒜素对副溶血弧菌抑菌作用的研究[J]. 公共卫生与预防医学,2017,28(1):130-132.
[23]刘婷,曹进,丁宏. 花椒籽提取物对副溶血弧菌抑菌作用的研究[J]. 食品安全质量检测学报,2017,8(2):634-638.
[24]蒋小强,邱红辉,符乃方,等. 红茶菌和中草药红茶菌体外抑制霍乱弧菌的研究[J]. 安徽农业科学,2015,43(7):4-6.
[25]李瑞,白东清,郭永军,等. 9种中草药及17种组方对迟钝爱德华氏菌的体外抑菌试验[J]. 水利渔业,2008,28(4):114-116.
[26]白东清,陈成勋,朱国霞,等. 抗生素对迟钝爱德华氏菌的体外抑菌试验[J]. 安徽农业科学,2010,38(3):1300-1302.
[27]詹能勇,李小勇,文明明. 43株酵母相马尔尼菲青霉菌纸片法药敏试验分析[J]. 国际检验医学杂志,2010,31(5):498-500.
[28]吴干斌,周建华,张军. 5种喹诺酮类药物体外抗菌活性的研究[J]. 中国临床药理学杂志,2014,30(2):129-130,159.
[29]苏碧仪,谭耀驹,吴爱武. 微孔板稀释法检测结核分枝杆菌丙硫异烟胺耐药结果分析[J]. 中国人兽共患病学报,2017,33(4):357-361.
[30]刘如运. 几种常用抑菌试验方法的评价及比较[J]. 现代企业教育,2013,433(14):341-342.
[31]郝贵杰,沈锦玉,潘晓义,等. 32种常用渔药对大黄鱼致病菌哈维氏弧菌的体外抗菌试验[J]. 中国兽药杂志,2008,42(5):36-39.
[32]樊海平,曾占壮,林煜. 盐酸恩诺沙星与其他四种抗菌药物对水产致病菌的体外抗菌作用[J]. 福建水产,2005(1):34-37.
[33]陈昌福. 水产养殖盲目用药危害大[J]. 当代水产,2010(8):30-31.
[34]杨先乐. 新编渔药手册[M]. 北京:中国农业出版社,2005:176-177.
[35]闻洋,陈寒嫣,杨炳君,等. 典型兽用抗生素对斑马鱼的急性毒性及氧化应激的影响[J]. 吉林师范大学学报:自然科学版,2018,39(2):94-98.
[36]杜冬冬,王涛,常维山. 五种抗生素联合应用对大肠杆菌抗菌效果实验[J]. 中国兽药杂志,2012,46(6):14-16.
[37]王成森. 磷霉素钙与头孢噻肟联用的体外抗菌活性试验[J]. 畜牧与兽医,2013,45(10):94-95.
[38]赵晓蕾,杨德红,严莲荷,等. 复配杀菌剂协同效应的研究[J]. 工业水处理,1999,19(6):31-32.
[39]孙英杰,孙红梅,邹洪波. 细菌耐药性产生的原因及防制措施[J]. 养殖技术顾问,2011(6):202-203.
[40]陈杖榴. 兽医药理学[M]. 北京:中国农业出版社,2002:250. |
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