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| Microbial Diversity in Intestinal Tract of Wild and Cultured Puffer Takifugu bimaculatus Based on 16S rRNA Gene Sequence |
| LEI Yang1, WANG Songgang1, CHEN Yu1, JIANG Yao1, ZHENG Yi1,2 |
1. College of Life Sciences, Fujian Normal University, Fuzhou 350117, China;
2. Southern Institute of Oceanography, Fujian Normal University, Fuzhou 350117, China |
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Abstract The V3—V4 regions of 16S rRNA were sequenced in the intestinal contents of wild puffer Takifugu bimaculatus collected from Tong'an sea area, Xiamen, China and cultured puffer by Through Illumina Miseq PE300 high throughput to explore the community structure of intestinal microorganisms. A total of 99500 superior sequences were obtained from six samples and the cluster analysis with 97% similarity showed that the number of OTU was 524 with unique OTU of 68 in wild puffer and 576 in cultured puffer with unique OTU of 120. Alpha diversity analysis was carried out from the calculation results of 4 indices (Chao1, observed-species, PD-whole-tree and Shannon). The results showed that there were higher diversity and richness of bacteria in cultured puffer than those in wild puffer. From the data in the phylum level Proteobacteria were of the dominant bacteria in wild and cultured puffer. Shewanella, Propionigenium, Photobacterium and Pseudoalteromonas producing tetrodotoxin were found in the wild puffer, and not found in the cultured puffer. The findings provided scientific evidence for exploring the toxicity-producing mechanism of tetrodotoxin, as the composition of dominant microflora was revealed in intestinal microorganisms of wild and cultured puffer.
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Received: 18 July 2019
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