红螯螯虾累代繁养群体的遗传多样性分析

doi:10.16378/j.cnki.1003-1111.2020.04.022

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1016 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract To evaluate the genetic diversity of red claw crayfish Cherax quadricarinatus in different breeding farms in China, 180 individuals of six breeding groups (Fuyang in Anhui, Huzhou in Zhejiang, Zhuhai in Guangdong, Zhenjiang in Jiangsu, Chengmai in Hainan, and Suzhou in Jiangsu) were cloned based on mitochondrial cytochrome oxidase subunit Ⅰ gene (COⅠ). Sequence alignment showed that there were 56 variable sites and 16 parsimony-informative sites in total 831 sites. The content of (G+C) was lower (39.765%), showing obvious bias of base composition. Ten haplotypes were detected among the 180 samples. Haplotype H1 found most frequently was shared by the populations from Fuyang, Zhuhai, Zhenjiang, Chengmai and Suzhou. Haplotype diversity index was 0.693 and nucleotide diversity index was 0.00652, indicating that the genetic diversity index of total samples was high. Suzhou population had the highest haplotype diversity index (0.964) while Chengmai population had the lowest haplotype diversity index (0.618) among the six populations. The genetic distance between Chengmai and Fuyang populations was higher than those of others while between Zhuhai and Suzhou is the lowest among the six red claw crayfish groups. The findings indicated that there was high level genetic diversity in red claw crayfish, there were also some gene exchanges among populations. This study has accumulated data for the development and utilization of domestic red claw crayfish germplasm resources.

Key words： Cherax quadricarinatus Genetic diversity COⅠ

Received: 01 July 2019

S917

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	PENG Gang
	XU Yu
	ZHANG Yan
	WANG Jing
	HUANG Hongbing
	YAN Weihui
	XU Zhiqiang

Cite this article:

PENG Gang,XU Yu,ZHANG Yan, et al. Genetic Diversity Analysis of Successive Generations of Red Claw Crayfish Cherax quadricarinatus in Different Breeding Areas in China[J]. Fisheries Science, 2020, 39(4): 615-619.

URL:

https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.2020.04.022 OR https://www.shchkx.com/EN/Y2020/V39/I4/615

[1]钟诗群,庞守忠. 红螯螯虾的生物学特性及其养殖简介[J]. 内陆水产,1997(8):25.
[2]李宁玉. 澳洲淡水龙虾的养殖前景与方法[J]. 海洋与渔业,2015(6):48-49.
[3]殷悦,严维辉,郑友,等. 红螯螯虾研究进展及在江苏的产业发展前景分析[J]. 水产养殖,2018,39(10):9-13.
[4]李庆勇,高文峰,候同玉,等. 澳洲淡水龙虾池塘健康养殖技术[J]. 中国水产,2017(4):90-92.
[5]Abinash P. Geographic variation within a tandemly repeated mitochondrial DNA D-loop region of a North American freshwater fish, Pylodictis olivaris[J]. Gene,2014,538(1):63-68.
[6]肖武汉,张亚平. 鱼类线粒体DNA 的遗传与进化[J]. 水生生物学报,2000,24(4):384-391.
[7]李晓晖,许志强,潘建林,等. 中华绒螯蟹人工选育群体的遗传多样性[J]. 中国水产科学,2010,17(2):236-242.
[8]廖健,龙水生,贺亮,等. 基于COⅠ基因和D-loop区部分序列比较雷州半岛东、西岸湖栖鳍虾虎鱼群体的遗传多样性[J]. 海洋科学,2017,41(2):103-110.
[9]郑芳,吕秀玲,孙红英,等. 基于线粒体COⅠ基因序列探讨长江华溪蟹的遗传分化[J].南京师大学报:自然科学版,2006,29(2):103-105.
[10]赵丹,刘莹,宋爱环,等.基于线粒体COⅠ序列的泥螺群体遗传多样性研究[J].水产科学,2017,36(3):353-358.
[11]郭健康,安苗,周其椿,等.喀斯特山区大眼鳜线粒体COⅠ基因序列分析[J].水产科学,2016,35(6):649-657.
[12]谢雁南. 红螯螯虾微卫星标记筛选及群体遗传学分析[D]. 上海:华东师范大学,2011.
[13]Skibinski D O F, Gallagher C, Beynon C M.Mitochondrial DNA inheritance[J]. Nature,1994,368:817-818.
[14]Awotunde E O, Bemji M N, Olowofeso O, et al. Mitochondrial DNA sequence analyses and phylogenetic relationships among two Nigerian goat breeds and the South African Kalahari Red[J]. Animal Biotechnology,2015,26(3):180-187.
[15]Nei M.分子群体遗传学和进化论[M]. 王家玉,译.北京:农业出版社,1975:121-133.
[16]李吉涛,段亚飞,李健,等. 脊尾白虾(Exopalaemon carinicauda)不同地理群体的生化遗传分析[J]. 海洋与湖沼,2013,44(2):360-365.
[17]Takuma S, Minoru I, Hideshi M, et al. Inheritance mode of microsatellite DNA markers and their use for kinship estimation in kuruma prawn Penaeus japonicas[J].Fisheries Science,2002,68(2):299-305.