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| Drug Resistance of Vibrio harveyi from Fishery Products in Wenzhou, China |
| WU Yue, ZHENG Yinuo, LU Rongmao, WANG Yaohua, YAN Maocang, HU Yuan, ZHOU Chaosheng |
| Zhejiang Mariculture Research Institute, Wenzhou 325005, China |
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Abstract Drug sensitivity analysis kit was used to test the minimum inhibitory concentrations of 14 drugs against 11 strains of Vibrio harveyi identified from the infected fishes, shellfishes and shrimps in maricultyre in Wenzhou, China by biochemical identification and 16S rRNA sequencing, and high-throughput quantitative RT-PCR assay was used to test the expression profile of resistance gane of sulfonamide, quinolone and tetracycline to analyze the drug resistance of V. harveyi from aquatic animals in marine in Wenzhou. Susceptibility test showed that the 11 strains of V. harveyi had the maximal resistance rates (100%) to sulfadiazine and ampicillin sodium, high resistance rates to sulfamethoxazole (as 99.30%) and sulfamethoxine (88.03%), and sensitive to sulfamethazine, ciprofloxacin, enrofloxacin and doxycycline. The three kinds of antibiotic resistance genes were detected positively in all strains, with the maximal detection rate of sul1 gene (81.82%) in all sulfonamide resistance genes, and the maximal detection rate of qnrD gene (63.64%) in all quinolone resistance genes, and the maximal detection rate of tet34, tet35 and tet36 (100%)in all tetracycline resistance genes. The findings indicated that most of the strains were multi-drug resistant to sulfonamides and β-lactam, and universally carried multiple resistance genes. There was a certain relationship between the sulfonamide resistance phenotype and the related resistance genes, while the quinolone, and tetracycline resistance phenotypes were not in direct proportion to the resistance genes.
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Received: 09 August 2019
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