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| Identification and Drug Resistance of Pathogenic Bacteria HY3 in Acute Hepatopancreatic Necrosis Disease from Pacific White Shrimp Litopenaeus vannamei |
| YU Dayong1,2, CHEN Bixiu1,2, ZHONG Yongjun1,2, LIANG Xiuyan1,2, HE Xinwei1,2, XIA Haiyang1, BAO Jiawei3 |
1. Institute of Biopharmaceuticals, Taizhou University, Taizhou 318000, China;
2. School of Life Sciences, Taizhou University, Taizhou 318000, China;
3. Zhejiang Hongye Seafood Co., Ltd., Linhai 317000, China |
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Abstract A pathogenic bacterium strain HY3 was isolated from Pacific white shrimp Litopenaeus vannamei with acute hepatopancreatic necrosis disease (AHPND) symptom collected in Taizhou fishery farm by streak plate method, and identified as Vibrio parahaemolyticus by the characteristics of colony on TCBS plate and the sequence analysis of 16S rRNA and fur gene, aiming to investigate the pathogen of AHPND of Pacific white shrimp and to propose prevention strategy. The HY3 strain had tlh (thermolabile hemolysin), pirAVP and pirBVP genes which caused shrimp AHPND. The pathogenic challenge test showed that the strain had strong pathogenicity, with cumulative mortality rate of nearly 100% for the shrimp on the seventh day after infection. Drug susceptibility test revealed that the strain was resistant to streptomycin and ampicillin. The resistance gene test indicated that related resistance genes blaTEM, strA and strB were detected in the strain by PCR and DNA sequencing. Potassium permanganate, a fishery disinfectant, showed strong bactericidal activity to the strain HY3, with bactericidal rate of 99.99% at 10 mg/L potassium permanganate for 2 h. V. parahaemolyticus was proposed as the main pathogen for the AHPND of Pacific white shrimp in Taizhou, with resistant to streptomycin and ampicillin which is not used in control AHPND in Taizhou. However, potassium permanganate is recommended to be applied as disinfectant to prevent AHPND in Taizhou.
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Received: 18 July 2019
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