贝莱斯芽孢杆菌MSP05对泥鳅生长、消化及免疫的影响

doi:10.16378/j.cnki.1003-1111.21127

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Abstract This study was conducted to investigate the effect of dietary Bacillus velezensis MSP05 on growth performance, digestion, and non-specific immune function of juvenile loach Misgurnus anguillicaudatus. 360 fishes with an average initial body weight of (3.24±0.14) g were randomly assigned into 4 groups in triplicates with 30 individuals per replicate. One control group and three experimental groups were added 0, 1×10⁶, 1×10⁷, 1×10⁸ cfu/g B. velezensis MSP05 to basal diets respectively. After 8 weeks of feeding, the results demonstrated that the weight gain rate(WGR) and specific growth rate(SGR) of the groups adding B. subtilis MSP05 were significantly higher than those of the control group (P<0.05). The activities of protease and amylase increased firstly and then decreased with the B. velezensis level increasing, achieving the highest value in 1×10⁷ cfu/g group, while the activities of lipase rose continuously. The optimal morphology of foregut, midgut and hindgut of loach were observed in 1×10⁶, 1×10⁶, 1×10⁷ cfu/g group, respectively. The activities of serum catalase (CAT), superoxide dismutase (SOD), lysozyme (LSZ) and alkaline phosphatase (ALP) in the serum were significantly higher than the control group (P<0.05), while MDA levels showed the opposite trend. Challenged by Aeromonas hydrophila for 15 days, the cumulative mortality rate of juvenile loaches decreased significantly fed with B. velezensis. In conclusion, the addition of B. velezensis MSP05 can significantly enhance the growth performance, digestive enzymes activity, intestinal morphology, antioxidant enzyme activity, non-specific immune function and disease resistance against A. hydrophila.

Key words： Misgurnus anguillicaudatus Bacillus velezensis growth performance digestive capacity non-specific immune

Received: 12 July 2021

S965.199

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	Articles by authors
	MENG Wenrong
	MI Haoyu
	HUANG Yichen
	HUANG Zhifa
	LIU Qitong
	XIA Mengjie
	FANG Yaowei

Cite this article:

MENG Wenrong,MI Haoyu,HUANG Yichen, et al. Effects of Dietary Bacillus velezensis MSP05 on Growth Performance, Intestinal Digestive Capacity and Non-Specific Immune of Loach Misgurnus anguillicaudatus[J]. Fisheries Science, 2023, 42(4): 712-718.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.21127 OR https://www.shchkx.com/EN/Y2023/V42/I4/712

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