低氧对鲂鲌杂交种F<sub>3</sub>鳃结构及生理生化的影响

doi:10.16378/j.cnki.1003-1111.21205

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Abstract By adjusting the amount of nitrogen or air in the water, hybrid F₃ of blunt snout bream Megalobrama amblycephala (♀) × culter Culter alburnus (♂) was exposed to normal [dissolved oxygen concentration = (7.16±0.20) mg/L], low oxygen [dissolved oxygen concentration = (1.04±0.12) mg/L] and recover [dissolved oxygen concentration = (7.19±0.23) mg/L]. Gills of hybrid F₃ of normal, hypoxia 12 h, hypoxia 24 h, hypoxia 72 h and recovery were used as samples. Hematoxylin-Eosin staining, Alcian blue-Periodie acid Schiff staining, antioxidant enzyme activity determination, qRT-PCR and other techniques were used to explore the adaptability of gill tissue of hybrid F₃ to hypoxia. The results showed that after 72 h of hypoxic treatment, the area of intercellular cell mass in gill tissue of hybrid F₃ was significantly reduced, the length of gill lamellae was prolonged, and the contact area between gill lamellae and water was increased, so as to adapt to hypoxic environment. The area of interlamellar cell mass was recovered after reoxygenation. Hypoxic stress led to significantly reduce the density of mucus cells in gill tissue of hybrid F₃, and reduce the secretion function of mucus in gill tissue. Under hypoxia stress, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in gill tissue of hybrid F₃ were the maximum at 72 h, and catalase (CAT) activity was the maximum at 24 h. Hypoxia stress significantly changed the mRNA expression levels of caspase-3 and Bcl-2 genes in gill tissue, with the different trends. In the 72 h hypoxia treatment group, the expression level of caspase-3 gene was the maximum and that of Bcl-2 gene was the minimum. After 24 h of reoxygenation treatment, the structure, physiological and biochemical indices of gill tissue was recovered. In summary, hypoxia environment significantly affected the morphological structure, mucus secretion function, and antioxidant enzyme activity of gill tissue of hybrid F₃, but did not completely destroy its self-regulation ability.

Key words： hybrid F₃ of Megalobrama amblycephala(♀) ×Culter alburnus (♂) hypoxia stress gill remodeling antioxidant enzyme apoptosis

Received: 11 October 2021

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	CHEN Songlin
	LIN Xin
	ZHENG Guodong
	ZOU Shuming

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CHEN Songlin,LIN Xin,ZHENG Guodong, et al. Effects of Acute Hypoxia Stress on Gill Structure, Physiology and Biochemistry of Hybrid F₃ of Blunt Snout Bream Megalobrama amblycephala ♀× Culter Culter alburnus ♂[J]. Fisheries Science, 2023, 42(2): 214-221.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.21205 OR https://www.shchkx.com/EN/Y2023/V42/I2/214

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