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| Karyotype and Ploidy Analysis of Gymnocypris selincuoensis |
| LI Xin1, MA Kexin1, GUO Wenxuan1, LI Yifan1, YANG Yueyao1, ZHOU Ziyu1, LIU Haiping2, XIAO Shijun3, ZHOU He1 |
1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;
2. Institute of Fisheries Science, Xizang Autonomous Region Academy of Agriculture and Animal Husbandry Sciences, Lhasa 850000, China;
3. Jiaxing Key Laboratory for New Germplasm Breeding of Economic Mycology, Jiaxing 314000, China |
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Abstract Harsh plateau environment in Tibetan Plateau has unique and rich biological resources. The chromosomes in different groups and genera in the subfamily Schizothoracinae showed diverse characteristics.The chromosome samples were prepared from renal tissue cells of Gymnocypris selincuoensis with body weight of 246.0—374.2 g by the conventional cold drop method. Giemsa staining analysis was used to determine the chromosome number and karyotype. The band type and ploidy were speculated through Ag-NORs and Chromomycin A3/4,6-diamidino-2-phenylindole double fluorescent staining technology. It was found that the karyotype formula of G. selincuoensis was 4n=88, 40m+20sm+28st/t, NF=148. In interphase nucleoli, the maximal frequency of Ag-NORs sites was 4. In each metaphase karyotype, both Ag-NORs sites and CMA3-positive sites were shown to be 4 on the short arms of submetacentric chromosomes. In conclusion, different G. selincuoensis samples had identical chromosome numbers. G. selincuoensis is a genetic tetraploid with four sets of chromosomes. The finding provides an important basis for studying genetic relationships among species and reference for the subsequent research on the classification and evolutionary status of the subfamily Schizothoracinae.
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Received: 09 December 2022
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