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| Effects of an Inhibitor Germanium Dioxide of Diatom Growth on Somatic Cell Induction System of Kelp Laminaria japonica |
| TIAN Pingping, LIU Yanling, CHEN Shuxiu, WANG Weiwei, LI Xiaojie, LI Yan |
| Key Laboratory of Algae Genetic Breeding and Cultivation Technology in Shandong Province, The Technological Innovation Center of Alga and Sea Cucumber in Shandong Province, National Alga and Sea Cucumber Engineering Technology Research Center, Shandong Oriental Ocean Sci-Tech Co. Ltd., Yantai 264003, China |
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Abstract In order to evaluate the inhibitory effect of the diatom inhibitor germanium dioxide (GeO2) on diatoms in the inducible system and effect on the inducible system, a tissue block with a side length of 0.2—0.3 cm in 1.0—1.5 cm length sporophytes of kelp Laminaria japonica as explants in 2% PESI culture medium was exposed to GeO2 at concentrations of 0, 1.00, 2.00, 2.50, 3.00, 3.50 and 4.00 mg/L in the 2% PESI medium to investigate the effect of GeO2 concentration on the incidence of kelp transparent mitoplast and callus induction rate in the induction system of the kelp. On the 80th day in the induction system, 1.00 mg/L of GeO2 was added to the diatom-contaminated system in the control group, and the medium was exchanged every 7—10 days to observe the therapeutic effect of GeO2 on the contaminative diatoms. The results showed that different concentrations of GeO2 had an extremely significant impact on the induction of somatic cells (P<0.01). On the 60th day of the induction experiment, the cell and branch numbers of mitoplast gradually were found to be decreased with the increase in GeO2 concentration, and almost no mitoplast was found in the groups of above 3.50 mg/L, without significant difference in the induction rates (above 80%) of transparent mitoplast among the low concentration groups (0—2.50 mg/L), distinctly higher than that in other high concentration groups(P<0.01).On the 80th day, no significant difference was found between 1.00 and 2.00 mg/L groups, and the introduction rates of callus were shown to be 83.33% and 73.61%, respectively, higher than that in other groups(P<0.01). The application of 1.00 mg/L GeO2 to treat contaminated samples showed excellent removal effectiveness of diatoms from the induction system of the kelp. In summary, the optimal concentration of GeO2 was 1.00 mg/L for the somatic cells induction system, which effectively inhibited diatom contamination without affecting the mitoplast and callus induction of the kelp.
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Received: 03 January 2024
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