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| Molecular Cloning and Expression of Activator Protein-1 in Small Abalone Haliotis diversicolor during Development and Bacterial Challenge |
| WANG Guodong,HE Simei,ZHANG Lili,WANG Yilei |
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Abstract Activator protein‐1 (AP‐1) is a transcription factor ,which is characterized by highly conserved dimeric leucine zipper DNA‐binding domain ,and participates in development and immune processes .In or‐der to evaluate the effect of AP‐1 gene in development and immune response ,the full cDNA of AP‐1 gene (HdAP‐1) was cloned in small abalone Haliotis diversicolor and challenged by Vibrio parahaemolyticus and expressed during development of the abalone by real time PCR .The full cDNA of HdAP‐1 was 1482 bp ,and 5′/3′untranslated region had 133/401 bp ,with a 948 bp open reading frame .The calculated mo‐lecular mass of encoded protein was 34 .83 ku and the predicted pI 9 .43 .HdAP‐1 contained Jun and bZIP domains ,a highly conserved domains of AP‐1 protein family .HdAP‐1 was detected in all examined tis‐sues .There was the maxmal expression level in hemolymph ,middle expression level in gill/kidney/diges‐tive tract/mucus gland ,the minimal level in hepatopancreas/mantle .There was a low expression level from fertilized eggs to blastula stage ,significantly lower than in gastrula stage (P<0 .05) .The expression of HdAP‐1 continuously increased from gastrula stage to trochophore stage ,with a high level from tro‐chophore to creeping larva stage ,and low level after metamorphosis .There was significant up‐regulation of HdAP‐1 24 h after V .parahaemolyticus challenge (P<0 .05) ,without significant difference between control and bacterial challenge in other phase (P>0 .05) .
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Received: 25 March 2016
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