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Artificial Propagation,and Embryonic and Yolk-sac Larval Development in Percocypris pingi in Yalong River |
DENG Longjun,GAN Weixiong,ZENG Rukui,ZHANG Hongwei |
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Abstract The wild Percocypris pingi was reared to spawn induced by injection of hormone to obtain fer‐tilized eggs in this study .The results showed that females injected with LHRH‐A2 +DOM+ HCG ovula‐ted more fluently ,with the response time of 58 h ,mean fertilization rate of 87 .33% and mean hatching rate of 90 .75% .Furthermore ,the embryonic and yolk‐sac larval development were described in detail . Under the water temperature of (16 ± 2) ℃ ,fertilized eggs were hatched in 117 .83 hours with the accumu‐lative temperature of 1855 .33 ℃ · h .The embryonic development was divided into 7 stages including 26 subphases :fertilized egg ,cleavage stage ,blastula stage ,gastrula stage ,neurula stage ,organogenesis stage and hatching stage .The yolk‐sac larva grew rapidly and endogenously by in the first five days after hatching ,turned into the stage of mixed nutrition ,a critical stage ,since the sixth day ,and started to swim horizontally ten days later .
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Received: 25 April 2016
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