|
|
|
| Detection of Rifampicin in Fishery Products by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with Dispersive Solid Phase Extraction |
| ZHU Sihua1, ZHANG Yukun1, LIU Zhenxian1, QIAN Baocong2, LI Bing2, MIAO Xiaoqun1 |
1.Foshan University of Science and Technology, Foshan 528000, China;
2.Guangdong Qualico Inspection Corporation, Dongguan 523000, China |
|
|
|
|
Abstract A method was established for the detection of rifampicin residues in fishery products by ultra performance liquid chromatography-tandem mass spectrometry with dispersive solid phase extraction. The samples were extracted with acetonitrile-dichloromethane (6∶4, V/V) , purified by a mixture of 100 mg C18 and 100 mg ethylenediamine-N-propylsilane (PSA), and then eluted with 3 mmol/L ammonium acetate solution (containing 0.05% formic acid). The electrospray ionization mass spectrometry was operated in the positive mode using multiple reaction monitoring (MRM), and the target compound was quantified by the internal standard method. The results showed that the calibration curve for rifampicin exhibited good linear relationship in the range of 1—20 μg/kg with correlation coefficients (r) of more than 0.9986, with detection limit of 0.3 μg/kg and the quantification limit of 1.0 μg/kg. The average recoveries for Nile tilapia hybrid Oreochromis aureus×O.niloticus, Pacific white shrimp Litopenaeus vannamei and Chinese soft shelled turtle Trionyx sinensis were ranged from 90.17%—101.07% with relative standard deviations (RSDs) of 3.11%—7.66%. The method is suitable for the detection and confirmation of rifampicin in fishery products due to its simplicity, rapidity, and high accuracy, and provides convenient and effective technical support for the supervision of illegal use of rifampicin.
|
|
Received: 21 January 2019
|
|
|
|
[1]张朋,李世明.利福平致严重不良反应文献复习[J].临床合理用药杂志,2015(19):91-93.
[2]张大贵,张丹.临床应用利福平应注意的几个问题[J].新医学,2002,33(5):304-305.
[3]Holmström K, Gräslund S, Wahlström A, et al. Antibiotic use in shrimp farming and implications for environmental impacts and human health[J]. International Journal of Food Science & Technology,2003,38(3):255-266.
[4]唐毓祎,谈建国,缪海振,等.南美白对虾养殖水体中霍乱弧菌的毒力、耐药及重金属抗性研究[J].上海海洋大学学报,2014,23(6):867-873.
[5]中华人民共和国农业部公告第560号[EB/OL].(2005-11-20)[2019-01-11].http:∥kns.cnki.net/KCMS/detail/detail.aspx?FileName=GNZB200511013&DbName=CJFN2005.
[6]李诗言,王扬,丁雪燕,等.水产品中利福平残留的超高效液相色谱串联质谱快速测定方法:中国,CN107941980A[P]. 2018-04-20.
[7]黄玉萍,邓玉婷,姜兰,等.复合水产养殖环境中气单胞菌耐药性及其同源性分析[J].中国水产科学,2014,21(4):777-785.
[8]吴雅丽,邓玉婷,姜兰,等.广东省水产动物源气单胞菌对抗菌药物的耐药分析[J].上海海洋大学学报,2013,22(2):219-224.
[9]蔡丽娟,许宝青,林启存.水产致病性嗜水气单胞菌耐药性比较与分析[J].水产科学,2011,30(1):42-45.
[10]全国兽药残留专家委员会办公室关于申报2018年兽药残留标准制修订项目的通知残留办〔2017〕28号[EB/OL].(2017-10-13)[2018-09-23].http:∥www.nmgsyxxw.cn/ws/news_play.asp?id=5102.
[11]Moschou I C, Dasenaki M E, Thomaidis N S. Ionization study and simultaneous determination of avermectins and milbemycines in fish tissue by LC-ESI-MS/MS[J]. Journal of Chromatography B,2019,11(4):134-140.
[12]万译文,黄向荣,伍远安,等.分散固相萃取/液相色谱串联质谱法测定水产品中磺胺类、喹诺酮类、青霉素类多种兽药残留[J].湖南师范大学自然科学学报,2018,41(3):52-57.
[13]李佩佩,张小军,梅光明,等. 分散固相萃取—超高效液相色谱—串联质谱检测水产品中14种喹诺酮类药物[J].食品科学,2014,35(24):265-270.
[14]杨霄,索纹纹,洪波,等.分散固相萃取—高效液相色谱—串联质谱法测定水产品中甲基睾酮的残留量[J].江苏农业科学,2018,46(12):150-152.
[15]程甲,赵善贞,霍忆慧,等.基质分散固相萃取—高效液相色谱—轨道离子阱高分辨质谱法对水产品中12种禁用化合物的快速筛查和确证[J].食品安全质量检测学报,2018,9(3):574-582.
[16]李佩佩,张小军,张帅,等.通过式固相萃取结合超高效液相色谱—四级杆飞行时间质谱筛查鱼肉中多类兽药残留[J].浙江海洋学院学报:自然科学版,2017,36(3):228-234. |
|
|
|
