斑点叉尾鮰败血症的病原学与病理组织学观察

doi:10.16378/j.cnki.1003-1111.2020.06.004

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (4675 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract A bacterial strain was isolated from liver and kidney of diseased channel catfish Ictalurus punctatus showing symptoms of septicemia in Neijiang city, Sichuan province, by traditional pathogen isolation methods in order to define the potential pathogens and disease control methods of the channel catfish. The pathogenicity of the strain was validated by infection test, and identified as Aeromonas hydrophila by morphological observation, physiological and biochemical characterization, and by 16S rRNA and gyrB gene sequence analysis. The antibiotic susceptibility indicated that the strain was sensitive to ofloxacin, norfloxacin, piperidic acid and tobramycin, intermediary to kanamycin, azithromycin and polymyxin B, and resistant to other drugs in the test. The histopathological observation of the diseased channel catfish showed that the diseased was characterized by congestion of hepatic lobular veins, hepatocyte granule degeneration, granular degeneration of hepatocytes vacuolar and even focal necrosis. Extensive congestion and hemorrhage of renal interstitium, lymphocyte infiltration, degeneration and exfoliation of renal tubular epithelial cells were observed in the diseased fish, and reduction in splenic lymphocyte number, congestion and hemorrhage were found as well. Furthermore, there were necrosis and exfoliation of intestinal mucosa and other pathological changes in the diseased channel catfish. It was concluded that A. hydrophila was identified as the pathogen causing the symptoms of septicemia. The findings provided theoretical basis for the prevention and treatment of the disease.

Key words： Aeromonas hydrophila Ictalurus punctatus isolation and identification pathohistological observation drug sensitivity

Received: 26 September 2019

S948

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Chengwei
	WANG Jun
	LIU Jiahui
	GENG Yi

Cite this article:

LI Chengwei,WANG Jun,LIU Jiahui, et al. Etiology and Pathohistological Observation of Septicemia in Channel Catfish Ictalurus punctatus[J]. Fisheries Science, 2020, 39(6): 813-819.

URL:

https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.2020.06.004 OR https://www.shchkx.com/EN/Y2020/V39/I6/813

[1]农业部渔业渔政管理局. 中国渔业统计年鉴2017[M]. 北京:中国农业出版社,2017:45.
[2]Abdelhamed H, Banes M, Karsi A, et al. Recombinant ATPase of virulent Aeromonas hydrophila protects channel catfish against motile Aeromonas septicemia [J]. Frontiers in Immunology,2019,10:1641.
[3]Wagner B A, Wise D J, Khoo L H, et al. The epidemiology of bacterial diseases in food-size channel catfish[J]. Journal of Aquatic Animal Health,2006,18(4):263-272.
[4]李刚. 斑点叉尾鮰套肠病的诊断与防治[J]. 中国水产,2015(10):84-85.
[5]李冠青,王艺,李槿年,等. 安徽部分湖泊养殖鱼类暴发性出血病的病原分离与鉴定[J]. 中国微生态学杂志,2011,23(6):496-499.
[6]胡安东,张明洋,张飘,等. 鲤源嗜水气单胞菌分离鉴定、耐药性及毒力基因检测[J]. 中国畜牧兽医,2019,46(7):2167-2175.
[7]叶彩燕,汪开毓,刘韬,等. 西伯利亚鲟致病性嗜水气单胞菌的分离鉴定[J]. 中国预防兽医学报,2018,40(3):195-199.
[8]乔毅,沈辉,万夕和,等. 异育银鲫源嗜水气单胞菌对磺胺类耐药性分析[J]. 水产科学,2018,37(4):456-463.
[9]周碧君,李永明,温贵兰,等. 斑点叉尾鮰致病性嗜水气单胞菌的分离[J]. 水产科学,2004,23(12):9-12.
[10]王友娟,李荣峰,李华,等. 辽宁地区养殖淡水鱼感染嗜水气单胞菌的流行病学调查[J]. 中国农业科技导报,2012,14(4):128-134.
[11]戴振炎,王荣华,李权生,等. 斑点叉尾鮰细菌性败血症的组织病理研究[J]. 湖南师范大学自然科学学报,2012,35(4):64-69.
[12]吴璇,袁海文,谢晓东,等. 斑点叉尾鮰套肠病病原嗜水气单胞菌的分离与鉴定[J]. 中国畜牧兽医,2017,45(10):3057-3062.
[13]童桂香,黎小正,韦信贤,等. 斑点叉尾鮰套肠症的病原鉴定及其药敏特性[J]. 大连水产学院学报,2009,24(6):475-481.
[14]Durborow R M, Hanson L. Severed intestine in channel catfish[J]. Journal of Wildlife Diseases,1988,24(1):146-149.
[15]汪开毓,耿毅,黄小丽,等. 斑点叉尾鮰传染性套肠症及其防治[J]. 淡水渔业,2006,36(6):61-63,65.
[16]梁万文,陈明,余晓丽,等. 斑点叉尾鮰肠败血症病原菌的分离与鉴定[J]. 西南农业学报,2007,20(5):1124-1129.
[17]刘玉林,王敏,王卫民. 斑点叉尾鮰病毒性疾病综述[J]. 水利渔业,2006,26(6):84-85,96.
[18]朱大玲,李爱华,钱冬,等. 嗜水气单胞菌毒力基因的研究进展[J]. 水生生物学报,2004,28(1):80-84.
[19]Ren Y, Li Y, Han G, et al. Research advances in drug resistance of Aeromonas hydrophila in fishery[J]. Chinese Journal of Biotechnology,2019,35(5):759-765.
[20]Khor W C, Puah S M, Koh T H, et al. Comparison of clinical isolates of Aeromonas from Singapore and Malaysia with regard to molecular identification,virulence, and antimicrobial profiles[J]. Microbial Drug Resistance,2018,24(4):469-478.
[21]李爱华,蔡桃珍,吴玉深,等. 我国鱼类病原——嗜水气单胞菌的耐药性研究[J]. 微生物学通报,2001,28(1):58-63.
[22]Ko W C, Yu K W, Liu C Y, et al. Increasing antibiotic resistance in clinical isolates of Aeromonas strains in Taiwan[J]. Antimicrobial Agents and Chemotherapy,1996,40(5):1260-1262.