胶红酵母分离鉴定及其对凡纳滨对虾幼体存活和变态的影响

doi:10.16378/j.cnki.1003-1111.19251

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Abstract Red yeast was isolated from the intestines of Pacific white shrimp Litopenaeus vannamei, tiger shrimp Penaeus monodon, mussel Perna viridis, scallop Mimachlamys nobilis and doctor fish Siganus fuscescens in Zhanjiang, Guangdong province, by streak plate method or dilution method, and the representative strains were identified by the 5.8S-ITS and 26S rRNA gene sequencing analysis after typing with the morphology and carbon source utilization characteristics. Then, the intermittent starvation stress test was conducted to compare the effect of the representative strains on survival of nauplius Ⅵ and ZoeaⅡof Pacific white shrimp to screen the optimal strains. Furthermore, the effects of the target strain on larval survival and metamorphosis were investigated with four administration modes including single low-dose, multiple low-dosage, single high-dosage, and multiple high-dosage inoculations from nauplius Ⅵ, as well as single high-dosage inoculation only at nauplius Ⅳ (early inoculation). The results showed that a total of 19 strains of red yeast were isolated and divided into six types according to the morphology and carbon source utilization characteristics, with six representative strains (designated as RY1—RY6). RY2, RY3, RY4 and RY5 were all identified as Rhodotorula mucilaginosa, while RY1 and RY6 were referred as R. diobovata and Cystobasidium slooffiae, respertively. Under intermittent starvation test, there was significantly higher survival rate of ZoeaⅡ exposed to strains RY1—RY5 than that in the control group (P<0.05), especially, significantly higher in RY4 group than in other strain groups (P<0.05). Significantly higher metamorphosis was observed in the zoea exposed to RY4 strain in three high-dose groups including the early, single, and multiply inoculation groups compared with the control group (P<0.05), without significant impact on larval survival for any of the treatment groups (P>0.05). Notably, there was a positive correlation between the larval survival of mysis Ⅰ and the yeast content in water under nutrient restriction condition. In conclusion, strain RY4 as R. mucilaginosa generally found in guts of marine economic animals from Zhanjiang can significantly improve the survival of Pacific white shrimp larvae under starvation stress. During shrimp larviculture, inoculating RY4 at multiple high-dose or at single high-dose at the early nursing stage such as nauplius Ⅳ can effectively promote larval metamorphosis, and larval survival under nutrient shortage.

Key words： marine red yeast Rhodotorula mucilaginosa identification Litopenaeus vannamei larva survival metamorphosis

Received: 09 November 2019

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	Articles by authors
	NING Weimin
	WEN Chongqing
	HUANG Xuemin
	LIU Jingwang
	CHEN Jieshi
	LYU Jiewei
	LIANG Huafang
	XUE Ming

Cite this article:

NING Weimin,WEN Chongqing,HUANG Xuemin, et al. Isolation, Identification and Effects of a Strain of Yeast Rhodotorula mucilaginosa on Survival and Metamorphosis of Larval Pacific White Shrimp Litopenaeus vannamei[J]. Fisheries Science, 2021, 40(1): 37-45.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.19251 OR https://www.shchkx.com/EN/Y2021/V40/I1/37

[1]Sathish Kumar T, Vidya R, Kumar S, et al. Zoea-Ⅱ syndrome of Penaeus vannamei in shrimp hatcheries[J]. Aquaculture,2017,479:759-767.
[2]黄雪敏,温崇庆,梁华芳,等. 健康和发病凡纳滨对虾糠虾期育苗池水体的菌群结构比较[J]. 广东海洋大学学报,2018,38(4):27-34.
[3]Wang A, Ran C, Wang Y, et al. Use of probiotics in aquaculture of China—a review of the past decade[J]. Fish & Shellfish Immunology,2019,86:734-755.
[4]Nagahama T, Hamamoto M, Nakase T, et al. Distribution and identification of red yeasts in deep-sea environments around the northwest Pacific Ocean[J]. Antonie Van Leeuwenhoek,2001,80(2):101-110.
[5]Yurkov A M, Kachalkin A V, Daniel H M, et al. Two yeast species Cystobasidium psychroaquaticum f. a. sp. nov. and Cystobasidium rietchieii f. a. sp. nov. isolated from natural environments, and the transfer of Rhodoto-rula minuta clade members to the genus Cystobasidium[J]. Antonie Van Leeuwenhoek,2015,107(1):173-185.
[6]刘雨,陈霞,宋琦,等.海洋红酵母的作用机理及其在畜禽水产养殖中的应用[J].饲料博览,2014(4):42-44.
[7]周泓,宛立,苏鹤声.海洋酵母简易培养新技术[J].水产科学,2002,21(6):24-25.
[8]陈昌福,姚鹃,肖雪生,等.海洋1号红酵母饲养日本对虾幼虾的效果[J].淡水渔业,2004,34(6):55-57.
[9]Yang S P, Wu Z H, Jian J C, et al. Effect of marine red yeast Rhodosporidium paludigenum on growth and antioxidant competence of Litopenaeus vannamei[J]. Aquaculture,2010,309(1/2/3/4):62-65.
[10]杨世平,吴灶和,简纪常.饲料中添加沼泽生红冬孢酵母对凡纳滨对虾消化酶及肠道微生物的影响[J].水产科学,2011,30(7):391-394.
[11]夏冬梅,杨铿,李卓佳,等.海洋红酵母对凡纳滨对虾生长及免疫的影响[J].广东农业科学,2013,40(14):133-137.
[12]Subramanian M, Alikunhi N M, Kandasamy K. Immunostimulatory effect of mangrove-derived marine yeasts in Penaeus monodon[J]. Aquaculture Research,2014,45(3):389-396.
[13]齐琼. 海洋红酵母的筛选及其在凡纳滨对虾养殖中应用效果初步研究[D].舟山:浙江海洋大学,2019.
[14]Nimrat S, Boonthai T, Vuthiphandchai V. Effects of probiotic forms, compositions of and mode of probiotic administration on rearing of Pacific white shrimp (Litopenaeus vannamei) larvae and postlarvae[J]. Animal Feed Science and Technology,2011,169(3/4):244-258.
[15]周德庆.微生物学实验手册[M].上海:上海科学技术出版社,1986:207-208,229-230.
[16]Kopsahelis N, Nisiotou A, Kourkoutas Y, et al. Molecular characterization and molasses fermentation performance of a wild yeast strain operating in an extremely wide temperature range[J]. Bioresource Technology,2009,100(20):4854-4862.
[17]Wang A R, Ran C, Ring E, et al. Progress in fish gastrointestinal microbiota research[J]. Reviews in Aquaculture,2018,10(3):626-640.
[18]Li E,Xu C,Wang X, et al. Gut microbiota and its modulation for healthy farming of Pacific white shrimp Litopenaeus vannamei[J]. Reviews in Fisheries Science & Aquaculture,2018,26(3):381-399.
[19]Von Doan H, Hoseinifar S H, Ring E, et al. Host-associated probiotics:a key factor in sustainable aquaculture[J]. Reviews in Fisheries Science & Aquaculture,2020,28(1):16-42.
[20]Kutty S N, Philip R. Marine yeasts—a review[J]. Yeast,2008,25(7):465-483.
[21]孙建男.雷州半岛近岸海域红酵母多样性分析及其代谢产物研究[D].湛江:广东海洋大学,2015.
[22]池振明,居靓,王祥红,等.在海洋环境中的酵母菌分布与多样性[J].中国海洋大学学报(自然科学版),2009,39(5):955-960.
[23]Zhou C, Lin H, Xia D, et al. Effect of dietary marine red yeast Rhodotorula mucilaginosa on the growth performance, and also non-specific immune responses of juvenile golden pompano Trachinotus ovatus when challenged with Vibrio harveyi[J]. Israeli Journal of Aquaculture-Bamidgeh,2016,68:1262.
[24]Cai Y, Yuan W, Wang S, et al. In vitro screening of putative probiotics and their dual beneficial effects:to white shrimp (Litopenaeus vannamei) postlarvae and to the rearing water[J]. Aquaculture,2019,498:61-71.
[25]梁华芳,欧黄思,吴耀华.凡纳滨对虾溞状幼体厌食症的防治[J].广东海洋大学学报,2013,33(6):87-91.
[26]温崇庆,赖心田,何红,等.两株对虾育苗用益生芽孢杆菌的筛选和鉴定[J].水生生物学报,2007,31(4):453-459.
[27]温崇庆,薛明,何红,等.蜡样芽孢杆菌对凡纳滨对虾幼体变态的影响[J].水产科学,2007,26(8):440-444.
[28]Hai N V. The use of probiotics in aquaculture[J]. Journal of Applied Microbiology,2015,119(4):917-935.
[29]Xue M, Wen C, Liang H, et al. In vivo evaluation of the effects of commercial Bacillus probiotics on survival and development of Litopenaeus vannamei larvae during the early hatchery period[J]. Aquaculture Research,2016,47(5):1661-1669.