盐藻钙调素基因的克隆及表达分析

doi:10.16378/j.cnki.1003-1111.20210

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Abstract In order to explore the structure and function of calmodulin gene in green alga Dunaliella salina, RT-PCR and RACE technology were used to clone D. salina calmodulin gene (DsCaM gene, GenBank accession number: MN428415). The DsCaM was then analyzed using bioinformatics method, and the expression of DsCaM gene under salt stress was detected by qRT-PCR method. The results showed that the full length of the cDNA of DsCaM gene was 1061 bp, the open reading frame was 495 bp, and it encoded 164 amino acids. DsCaM protein was hydrophilic and mainly distributed in cytoplasm and vacuoles, It contained no transmembrane area and signal peptide. The secondary structure of this protein was mainly α-helix (56.71%) and random coils (26.22%). Further phylogenetic analysis showed that DsCaM gene and Chlamydomonas reinhardtii CaM genes were closely related. The results of qRT-PCR showed that the expression of DsCaM gene was significantly up-regulated under high salt stress, the maximum at 6 h (P<0.01). The findings take a significant role in further analysis of functions of the D. salina calmodulin gene and the molecular pathways under salt stress.

Key words： Dunaliella salina calmodulin (CaM) qRT-PCR bioinformatics analysis

Received: 27 August 2020

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	Articles by authors
	WANG Mingfang
	GAO Xiangnan
	XU Weiwei
	CONG Yuting
	CHAI Xiaojie

Cite this article:

WANG Mingfang,GAO Xiangnan,XU Weiwei, et al. Cloning and Expression Analysis of CaM from Green Alga Dunaliella salina[J]. Fisheries Science, 2021, 40(3): 409-415.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20210 OR https://www.shchkx.com/EN/Y2021/V40/I3/409

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