甲基睾酮暴露对鳗鲡精巢发育的影响

doi:10.16378/j.cnki.1003-1111.20097

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Abstract The male Japanese eel Anguilla japonica with body weight of (266.45±18.65) g was held in a round culture bucket with a diameter of 1.5 m, a height of 1.0 m and a water volume of 1000 L at a rate of 10 fish and exposed to 17α-methyltestosterone concentration of 50 μg/L and to 5 mL of absolute ethanol in the control group at water temperature of (21±1) ℃ and a salinity of 35 for 45 d, without feeding, and with half exchange of the water every 5 days. After 17α-methyltestosterone exposure, the histological sections of Japanese eel testis were examined and serum 11-ketotestosterone (11-KT) and estradio (E2) levels were determined by ELISA to investigate the promoting effect of 17α-methyltestosterone on gonadal development and possible mechanism. The results showed that the male Japanese eel exposed to 17α-methyltestosterone concentration, with white and flaky testis at phase Ⅳ/ spermiogenesis stage (S5), had gonado-somatic index of (1.63±0.68)%, significantly higher than the eel in the control (about 8 folds) did, with thin testis with serrated edge at phase Ⅱ/ spermatogonial proliferation stage (S1) in the controls group. There was significantly higher serum E2 level in the male Japanese eel exposed to 17α-methyltestosterone [(182.06±30.52) pg/mL] than that in the control group (P<0.05), without significant change in serum 11-KT level between the male Japanese eel exposed to 17α-methyltestosterone [(7.30±2.15) pg/mL] and male Japanese eel in the control group, indicating that MT might induced spermatogenesis in Japanese eel by exposure.

Key words： Anguilla japonica 17α-methyltestosterone (MT) 11-ketotestosterone (11-KT) estradio (E2)

Received: 08 May 2020

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	LAI Xiaojian
	PENG Shuai
	ZHANG Zhe
	LI Zhongqin

Cite this article:

LAI Xiaojian,PENG Shuai,ZHANG Zhe, et al. Testis Development Induced in Japanese Eel Anguilla japonica by 17α-Methyltestosterone Exposure[J]. Fisheries Science, 2021, 40(6): 900-904.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20097 OR https://www.shchkx.com/EN/Y2021/V40/I6/900

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