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| Effects of Compound Probiotics on Intestinal Microflora of Pacific White Shrimp Litopenaeus vannamei |
| HAO Shuang1, ZHANG Zhenguo2, YOU Hongzheng1, MA Lin1, TANG Hongxia3, LIU Xiaolian1, XU Xiaoli1, GU Dexian1, LUO Zhang1 |
1.Tianjin Fishery Research Institute, Tianjin 300221, China;
2.Tianjin Animal Disease Prevention and Control Center, Tianjin 300402, China;
3.Tianjin Agricultural University, Tianjin 300384, China |
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Abstract In order to analyze the effect of compound probiotics (Bacillus subtilis and Lactococcus lactis) on the community structure of intestinal microflora and disease resistance of Pacific white shrimp Litopenaeus vannamei, Pacific white shrimp were fed diet containing probiotics. The intestinal microflora of Pacific white shrimp were determined by high-throughput sequencing method, and the cumulative mortality was measured after the shrimp was infected artificially by Vibrio parahaemolyticus within 14 days. The results showed that the range of OTU was 393—872, and that the number of OTU, observed species index, Chao1 index and Shannon index in intestinal tract of shrimp were relatively high in test group at the 28th and the 56th days. At the level of phylum classification, there were high proportion of Proteobacteria, Bacteroidetes and Actinobacteria in the intestinal microflora of Pacific white shrimp in the test group and control gruop. Compared to the control group, the proportion of Actinobacteria and Firmicutes was increased in the test group at the 56th day. At the level of genus classification, compared to the control group, the proportion of Ruegeria was increased, whereas the proportion of Vibrio, Pseudoalteromonas, and Tenacibaculum was decreased in the test group; PCoA analysis and UPGMA analysis showed that the microbial communities in control group on the 28th and the 56th days were similar, but significantly different from those in the test group on the 28th day. The cumulative mortalities of the test group and control group were (64.4±11.7)% and (81.1±5.1)% under the challenge of V. parahaemolyticus, and the survival rate of shrimp was increased by 16.7% in the compound probiotics. In conclusion, feeding compound probioties could magnify the abundance of intestinal microflora of Pacific white shrimp, inhibit the growth of opportunistic pathogen, and improve intestinal bacterial group composition of the shimp, and the ability of shrimp to resist to V. parahaemolyticus infection. After 28 days of stopping feeding the compound probiotics, there was still continous effect on the intestinal microflora. The finding provides useful reference for the application of compound probiotics in actual culture.
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Received: 04 December 2020
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