2种抗雌激素药物对雄性中华鳖脂代谢的影响

doi:10.16378/j.cnki.1003-1111.20283

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Abstract We investigated the effects of estrogen deficiency on lipid metabolism in Chinese soft-shelled turtle Pelodiscus sinensis by intraperitoneal injection of different concentrations of Letrozole (LET) and Fulvestrant (ICI) to detect differences in the expression of Cyp19a1 genes related to lipid metabolism and liver histology. Liver staining deepened in stained histological sections after ICI treatment, indicating that fat accumulation was produced in the liver and that this accumulation was dose-dependent. RT-qPCR results showed that Cyp19a1 expression level was significantly lower with the increase in treatment concentration (P<0.05) at 1 d after ICI treatment, significantly higher (P<0.05) at 3 d and 42 d. Similarly, as the ICI treatment concentration increased,the expression levels of Fas, Scd,Pparγ and Stat3 genes were significantly higher (P<0.05). Cyp19a1 expression level was significantly higher (P<0.05) after 42 d of treatment with low concentrations of LET and significantly lower (P<0.05) with high concentrations of LET. The expression level of Fas gene was increased with the increase in treatment concentration and was significantly lower in the 49 d in high concentration group (P<0.05). In the 49 d, the expression levels of Scd and Pparγ were decreased in treatment concentration group, and the expression level of Stat 3 was increased in high concentration group. RT-qPCR results revealed that disturbances in estrogen metabolism led to dysregulation of lipid metabolism and were associated with estrogen concentrations. The findings provided a theoretical reference for discussing the relationship between estrogen and lipid metabolism and breeding in the soft-shelled turtle.

Key words： Pelodiscus sinensis estrogen lipid metabolism letrozole fulvestrant

Received: 24 December 2020

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	Articles by authors
	WANG Guiyu
	WANG Luming
	WU Qisheng
	CEN Shuangshuang
	MA Xiao
	LI Xuejun

Cite this article:

WANG Guiyu,WANG Luming,WU Qisheng, et al. Effects of Letrozole and Fulvestrant on Lipid Metabolism in Male Chinese Soft-Shelled Turtle Pelodiscus sinensis[J]. Fisheries Science, 2023, 42(1): 65-72.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20283 OR https://www.shchkx.com/EN/Y2023/V42/I1/65

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