金边鲤肌球蛋白结合蛋白C3基因的克隆与表达分析

doi:10.16378/j.cnki.1003-1111.21057

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Abstract In this study, the structure and organizational expression characteristics of myosin binding protein C3 (MyBPC3) gene were investigated in Jinbian carp Cypinus carpio with body weight of (74.74±0.92) g. Functions of MyBPC3 on regulation mechanism of skin color variation were analyzed. The full-length cDNA sequence of MyBPC3 gene was cloned by RACE technique, and its tissue expression and correlation with melanin synthesis related genes (TYR, TYRP1, TYRP2 and MC-1R) were analyzed. The results showed that the full length cDNA of MyBPC3 gene was 4253 bp, including an open reading frame (ORF, 3783 bp) encoding 1260 amino acids. The predicted protein had molecular weight of 141.57 ku, the theoretical isoelectric point of 6.42, and 22 PKC and 5 PKA potential phosphorylation sites. Phylogenetic tree and homology analysis showed that the MyBPC3 gene of Jinbian carp was the most closely related to that of crucian carp Carassius auratus. The qRT-PCR results indicate that MyBPC3 mRNA is distributed in different tissues of Jinbian carp, with significantly higher expression in heart than that in brain, skin, muscle, spleen, liver, gut, and kidney tissues, and significantly lower expression level in heart and skin tissues of the carp with yellow skin individuals than that in the black one (P<0.05). The correlation analysis revealed that the MyBPC3 gene was significant positively correlated with melanin synthesis related genes (TYR, TYRP1, TYRP2 and MC-1R). In conclusion, MyBPC3 gene might involve in melanin synthesis of the skin, and its expression difference might be the cause of the skin color variation of Jinbian carp, which provided a reference for the study of the differential regulation mechanism of skin pigmentation in Jinbian carp.

Key words： Cypinus carpio body color MyBPC3 gene cloning expression analysis

Received: 09 April 2021

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	Articles by authors
	WEI Lingjing
	LYU Yejian
	GAN Baojiang
	HUANG Jie
	LIU Kang
	ZHANG Sheng
	TENG Zhongzuo
	MO Feilong
	YE Xiangchen

Cite this article:

WEI Lingjing,LYU Yejian,GAN Baojiang, et al. Cloning and Expression Analysis of Myocardial Globulin Binding Protein C3 (MyBPC3) of Jinbian Carp Cypinus carpio[J]. Fisheries Science, 2023, 42(4): 648-656.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.21057 OR https://www.shchkx.com/EN/Y2023/V42/I4/648

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