卡拉胶对七彩神仙鱼生长、酶活性和肠道微生物组成的影响

doi:10.16378/j.cnki.1003-1111.22054

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Abstract In order to evaluate effects of carrageenan on growth, digestive enzyme activity, antioxidant enzyme activity and intestinal microbial composition of discus fish Symphysodon haraldi, the discus fish with initial body weight of (10.81±2.41) g was reared in 80 L breeding tanks at stocking density of 15 individuals per tank, and fed diet containing 0% (and beef heart burger as controls), 3%, 6%, 9%, 12% and 15% carrageenan at water temperature of (28.0±0.5) ℃ for 56 days. The results showed that the maximal weight gain rate and specific growth rate were observed in 3% carrageenan group. There were significantly higher pepsin activity and foregut amylase activity in 3% carrageenan group than those in other groups, and significantly lower liver malondialdehyde level and glutathione peroxidase activities than other experimental groups. The activity of superoxide dismutase was significantly higher in liver of the discus fish fed the diet containing 12% of carrageenan than that in other experimental groups. The relative abundance of Proteobacteria was significantly higher in intestinal microbial composition in 3% carrageenan group than that in other groups at phylum level, and the relative abundance of Fusobacteria decreased. In terms of genus level, there was no Lactococcus in the beef heart burger group, significantly higher content of Rombuutsia in 3% carrageenan group than that in other carrageenan groups, and significantly higher content of the Cetobacterium than that in other groups. It was found that addition of 3% carrageenan improved the weight gain rate and specific growth rate of disc fish, significantly increased the activity of pepsin and foregut amylase, without oxidative stress, and with increase in relative abundance of Proteus, Rombudsia and Lactococcus. Under the conditions of this experiment, 3% of carrageenan is of the appropriate dietary amount for discus fish.

Key words： Symphysodon haraldi carrageenan growth digestion and immunity intestinal microbial composition

Received: 20 June 2022

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	Articles by authors
	ZHANG Haoran
	WEN Bin
	PAN Yunchao
	YANG Botian
	GAO Jianzhong
	CHEN Zaizhong

Cite this article:

ZHANG Haoran,WEN Bin,PAN Yunchao, et al. Effects of Carrageenan on Growth, Enzyme Activities and Intestinal Microbial Composition of Discus Fish Symphysodon haraldi[J]. Fisheries Science, 2024, 43(3): 400-409.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.22054 OR https://www.shchkx.com/EN/Y2024/V43/I3/400

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