藻粉对宽体金线蛭生长、酶活性和基因表达的影响

doi:10.16378/j.cnki.1003-1111.23061

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Abstract In order to reveal the effects of alga Chlorella pyrenoidosa and Spirulina platensis meals as assistant diet on the growth, digestive enzyme and antithrombin activities, as well as related gene expression of leech Whitmania pigra, leeches with body weight of (4.15±0.03) g were randomly placed into transparent plastic boxes of each 8.5 cm×27.5 cm×13.5 cm at density of 20 individuals per box, and were fed snail S. quadrata (snail group) once a day, the snail + C. pyrenoidosa powder (chlorella powder group) and the snail + S. platensis (spirulina powder group) once every other day with triplicate for 30 days. The digestive tract tissues were dissected, frozen with liquid nitrogen, and the expressions levels of growth-related genes such as growth hormone (GH) and insulin-like growth factor-1 (IGF1), and expression levels of α α-glucosidase (α-GLU), adipose triglyceride lipase (ATGL), protease (PT) digestive enzyme-related genes, and expression levels of immune-related genes superoxide dismutase (SOD) and hirudin gene (WP) were detected at the end of the experiment by MyiQ2 Two Color real-time quantitative PCR (Bio-Rad). The results showed that there was significantly higher body weight in chlorella powder group (P<0.05) in 10 d, 20 d and 30 d, significantly higher specific growth rate than that in snail group in 10 d, and significantly higher than that in spirulina powder group in 25 d and 30 d (P<0.05) . The leech had significantly higher food intake in chlorella powder group than that in other groups in 30 d (P<0.05), with significantly lower food conversion ratio than that in snail group in 10 d, and in spirulina powder group in 30 d (P<0.05). The survival rate was found to be over 91.67% in all groups. Moreover, significantly higher antithrombin activity was observed in spirulina powder group and chlorella powder group than that in snail group in 30 d (P<0.05), with significantly higher lipase activity in chlorella powder group than that in the other groups in 30 d (P<0.05). There were significantly higher expression levels of GH, IGF1 and PT genes in chlorella powder group than that in spirulina powder group in 30 d, while the significantly higher relative expression level of GH and PT genes in the chlorella powder group than that in snail group in 30 d (P<0.05) . In conclusion, C. pyrenoidosa meal as assistant diet in combined with S. quadrata led to significantly improve the growth performance, enhance the activities of lipase and anticoagulant, and affect related gene expression of W. pigra after 30 d feeding.

Key words： Whitmania pigra Chlorella pyrenoidosa meal Spirulina platensis meal growth and digestive enzyme antithrombin

Received: 16 August 2023

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	YAN Jingnan
	WU Yirong
	ZHANG Yuting
	JIANG Lingli
	GAO Youling

Cite this article:

YAN Jingnan,WU Yirong,ZHANG Yuting, et al. Alga Meals Affect Growth, Enzyme Activities, and Gene Expression of Leech Whitmania pigra[J]. Fisheries Science, 2024, 43(6): 974-983.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.23061 OR https://www.shchkx.com/EN/Y2024/V43/I6/974

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