克氏原螯虾循环养殖池塘微生物组成的研究

doi:10.16378/j.cnki.1003-1111.24026

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Abstract In order to probe into the structural composition of the aquatic microbial community in a recirculating red swamp crayfish Procambarus clarkii pond culture model based on the application of organic fertilizers, the microbial structure in the recirculating crayfish ponds was compared with traditional culture crayfish ponds and integrated rice-crayfish system in Wanhe Breeding Cooperative, Wuwei City, Anhui Province (E 117°93'32″, N 31°39'84″) from July to September by Illumina Miseq high-throughput sequencing technology. The results showed that the dominant bacterial communities in water in the three modes were similar at phylum level, and different in the dominant genera which were all colonies beneficial to carbon, nitrogen, and phosphorus recirculating in the water. The microbial communities in the three modes played promoting role in the balance of aquaculture water ecosystems. High flux sequencing analysis of the microorganisms in three periods in water in the middle and late stages of recirculating crayfish ponds showed that hgcI_clade was the core dominant bacterial genus, with relative abundance exceeding 20% in all three periods. The fish in the aquaculture water was found to affect the composition of the water microbial community. In August, the removal of miscellaneous fish from the ponds and a new round of water replacement resulted in significant difference in the structure of the water microbial community compared to July. As the aquaculture in the recirculating model progressed, the distribution of microbial structure in the water in September was closer to that in July. The most dominant bacteria in the aquaculture crayfish ponds wete found to be Proteobacteria, which played an important role in nutrient recirculating in the water. This may be involved in the aquaculture pond recirculating mode. The water physical and chemical factors showed that dissolved oxygen, PO^3-₄-P and NH₃-N were shown to be the environmental factors primarily impacting on the microbial structure of recirculating aquaculture crayfish ponds with C39, the dominant bacterial genus in recirculating aquaculture crayfish ponds playing a dominant role in nitrogen and phosphorus recirculating in the water.

Key words： Procambarus clarkii intensive crayfish pond recirculating aquaculture system microbial community

Received: 05 February 2024

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S917.1

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	Articles by authors
	LU Xinran
	DING Zhenyuan
	LI Fuhao
	WAN Changjian
	GUAN Weibing

Cite this article:

LU Xinran,DING Zhenyuan,LI Fuhao, et al. Microbial Composition in Water of Recirculating Aquaculture Ponds of Red Swamp Crayfish Procambarus clarkii[J]. Fisheries Science, 2025, 44(3): 341-355.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24026 OR https://www.shchkx.com/EN/Y2025/V44/I3/341

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