牟氏角毛藻藻际细菌的分离鉴定及其菌藻共培养体系的生长效应

doi:10.16378/j.cnki.1003-1111.24096

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Abstract Epiphytic bacteria will promote growth and the accumulation of nutrients in the microalgal cells. In order to construct the symbiosis system of Chaetoceros muelleri and establish the rapid and stable propagation technology of C. muelleri, 1 mL of concentrated bacterial solution was added to the basic algae solution of the co-culture system of C. muelleri at algal cell density of 4.16×10⁵ cells/mL during the aseptic growth phase, and the total bacterial concentrations of 10⁶, 10⁷ and 10⁸ cfu/mL, respectively, and 1mL of NMB3 was added to the control group. The associated bacteria were isolated, identified using 16S rDNA and purified using the streak plate method. The changes in algal cell density, and chlorophyll-a content were monitored every 2 days during 10 days co-culture, and contents of dry weight, total polysaccharide, and total protein were determined on the 10th day. The results showed that two strains of epiphytic bacteria were isolated and identified from the C. muelleri culture medium, including strain M01 Algoriphagus marincola, and strain M02 Halomonas piezotolerans. Both strains led to increase in the biomass of C. muelleri in the co-culture system, and in elevated chlorophyll-a content of C. muelleri at concentrations of 10⁶ cfu/mL and 10⁷ cfu/mL for M01, with the maximal cell density (3.33×10⁶ cells/mL) at a concentration of 10⁸ cfu/mL throughout the entire culture period for M02 by the end of the culture, 19.28% higher than that in the control group (P<0.05). The chlorophyll-a content was found to be increased for M02 by 17.83%, reaching 3.51 μg/L (P>0.05). Both M01 and M02 resulted in improvement of accumulation of total protein in C. muelleri, with the maximal total protein content at 10⁶ cfu/mL, 27.52% for M01 (P<0.05) and 11.39% for M02 (P>0.05) higher than that in the control group. However, both M01 and M02 did not significantly affect the dry weight or polysaccharide content of C. muelleri. In conclusion, the co-culture system with the two epiphytic bacterial strains improved both the growth efficiency and overall health of C. muelleri.

Key words： Chaetoceros phycosphere bacterium bacter-algal symbiotic isolation

Received: 05 June 2024

PACS:

S968.213

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	Articles by authors
	HUANG Jin
	GUO Yongjun
	LIU Huiru
	LIANG Shuang
	LI Yongren
	ZHANG Tao
	WANG Donghao
	NIE Yuxuan
	LIANG Jian

Cite this article:

HUANG Jin,GUO Yongjun,LIU Huiru, et al. Isolation and Identification of Bacteria in Phycospher from Microalga Chaetoceros muelleri and Growth Effect in a Bacterial-Algal Co-Culture System[J]. Fisheries Science, 2025, 44(3): 365-374.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24096 OR https://www.shchkx.com/EN/Y2025/V44/I3/365

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