海洋红酵母的鉴定筛选及CM6菌株对仿刺参幼参生长的影响

doi:10.16378/j.cnki.1003-1111.24043

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Abstract In order to obtain the marine red yeast suitable for cultivation of sea cucumber Apostichopus japonicus, the morphological observation, biochemical analysis, molecular biology identification, determination of metabolite content and the effect of feeding the sea cucumber were carried out. The juvenile sea cucumber with body weight of (0.81±0.02) g were reared in a 720 mm×490 mm×380 mm aquarium at density of 50 individuals and fed the basic diet with a concentration of 10⁷ cfu/g suspension CM6 yeast (test group), the basic diet containing marine red yeast CICC31029 (positive control group) and only the basic diet. The results showed that these strains of yeasts were belonged to Rotorua mucilaginosa, the strain CM6 exhibiting the best growth performance and the maximal contents of vitamin E, carotenoids and astaxanthin. By the 5th day of culture, the sea cucumber in the test group had 2.8 times higher biomass than those in the control group, and 22.3% and 71.6% higher contents of vitamin E and astaxanthin than those in the blank control group and positive control group, respectively. There were significant increase in specific growth rate and digestive enzyme activities in the test group and positive control group than those in the blank control group (P<0.05). Meanwhile, different qualities of Rotorua mucilaginosa had different effects on the growth performance of juvenile sea cucumber. The increase in specific growth rate by 25.7% and in the activities of amylase and trypsin in the intestines to some extent were observed in the juvenile sea cucumber in the test group compared with the positive control group, which might be involved in CM6 containing more active metabolites in the test group. The findings indicate that addition of an appropriate amount of yeast CM6 to the feed leads to increase the digestive enzyme activity and specific growth rate of juvenile sea cucumber.

Key words： marine red yeast fermentation sea cucumber juvenile growth astaxanthin

Received: 28 March 2024

PACS:

S968.9

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	Articles by authors
	YE Bo
	ZHAO Zhenjun
	LIU Danni
	WANG Xuda
	YANG Boxue
	DONG Ying
	WANG Xiaoyue
	LIU Guilin
	ZHANG Qian
	LI Shilei

Cite this article:

YE Bo,ZHAO Zhenjun,LIU Danni, et al. Identification of Four Strains of Marine Red Yeasts and Effect of Strain CM6 on Growth of Juvenile Sea Cucumber[J]. Fisheries Science, 2025, 44(3): 375-384.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24043 OR https://www.shchkx.com/EN/Y2025/V44/I3/375

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