海水酸化对褐牙鲆消化和免疫的影响

doi:10.16378/j.cnki.1003-1111.24136

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Abstract To investigate the effects of seawater acidification on activities of digestive enzymes and immune enzymes and gene expressions in bastard halibut Paralichthys olivaceus, juvenile bastard halibut with body weight of (2.16±0.34) g were reared in a glass tank (1.0 m×0.7 m×0.7 m) in the Key Laboratory of Northern Marine Aquaculture, Ministry of Agriculture and Rural Affairs, Dalian Ocean University for 49 d under natural light and water temperature of (19.00±1.50) ℃. CO₂ was added to the seawater to keep the pH at 7.90, 7.70, 7.50 and 7.30, and natural seawater (pH 8.10) were used as the control group. The activities and genes expression levels of pepsin (PES) and trypsin (TRS) in stomach, lipase (LPS) in intestine, catalase (CAT), acid phosphatase (ACP), alkaline phosphatase (ALP), and total superoxide dismutase (T-SOD) in muscle were determined at the end of the feeding trail. The results showed that the activities of PES, TRS, ACP, ALP, CAT, and T-SOD) were decreased with decreasing pH in Paralichthys olivaceus, with increase in LPS with decreasing pH and without significant differences in the activities of TES, TRS, LPS, ACP, and T-SOD compared to the control group from day 1 to day 14 in pH 7.50 and pH 7.30 groups(P>0.05).There were significantly lower (or higher) activities of the above enzymes than those in the control group from day 35 to day 49 (P<0.05); ALP and CAT activities were significantly lower (or higher) than those in the control group during the experiment (P<0.05). During the experiment, the minimal activities were observed 8.75 U/g in PES, 138.81 U/g in TRS, 0.009 U/g prot in ACP, 0.019 U/g in ALP, and 17.46 U/g in SOD at the 49 day in the pH 7.30 group. The maximal activity (14.534 U/g) of LPS was found in the pH 7.30 group at 49 days. The relative expression levels of digestive genes prss1 and cpa4, as well as immune genes pik3r5 and cxcl8a were found to be decreased in the muscles with decreasing pH, significantly lower than those in the control group from 35 to 49 days (P<0.05), indicating that the digestive enzyme and immune enzyme activities, as well as the expression of digestive and immune genes, of P. olivaceus are significantly affected by high-intensity acidification environment. Therefore, seawater acidification environment will affect the stability of P. olivaceus population in natural sea areas.

Key words： Paralichthys olivaceus seawater acidification digestive enzyme immune enzyme gene expression

Received: 16 August 2024

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S965.399

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	CHANG Yifan
	XUE Jiaxin
	LIANG Guangyan
	ZHANG Zelin
	WEN Jin
	YUAN Jun
	YU Xiaoming
	CHEN Lei

Cite this article:

CHANG Yifan,XUE Jiaxin,LIANG Guangyan, et al. Effect of Seawater Acidification on Digestion and Immunity of Bastard Halibut Paralichthys olivaceus[J]. Fisheries Science, 2025, 44(4): 511-522.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24136 OR https://www.shchkx.com/EN/Y2025/V44/I4/511

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